DIAPH-2 Antibody (CL1113) - BSA Free

Western Blot: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.

Immunohistochemistry: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human kidney shows moderate positivity in renal glomeruli.

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human pancreas shows absence of immunoreactivity (negative control).

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human placenta shows strong staining in the trophoblast.

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.

• Reactivity: Hu
• Applications: WB, IHC, Mycoplasma
• Clone: CL1113
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

DIAPH-2 Antibody (CL1113) - BSA Free Summary

• Description: Novus Biologicals Mouse DIAPH-2 Antibody (CL1113) - BSA Free (NBP2-52967) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O60879, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DIAPH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  DIAPH-2 Recombinant Protein Antigen (NBP2-52967PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for DIAPH-2 Antibody (CL1113) - BSA Free

• DIA2
• DIAdiaphanous (Drosophila, homolog) 2
• DIAPH2
• DIAPH-2
• diaphanous homolog 2 (Drosophila)
• Diaphanous-related formin-2
• diaphorase-2
• DRF2
• POF
• POF2
• POF2FLJ11167
• protein diaphanous homolog 2

Background

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIAPH2