DIAPH3 Antibody (4D5)

Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: DIAPH3 Antibody (4D5) [H00081624-M01] - Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, S-ELISA
• Clone: 4D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DIAPH3 Antibody (4D5) Summary

• Immunogen: DIAPH3 (NP_112194, 632 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKV
• Specificity: DIAPH3 - diaphanous homolog 3 (Drosophila) (4D5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DIAPH3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DIAPH3 Antibody (4D5)

• DIAP3
• diaphanous homolog 3 (Drosophila)
• diaphanous-related formin 3
• DKFZp434C0931
• DKFZp686A13178
• homolog) 3
• mDia2
• protein diaphanous homolog 3

Background

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIAPH3
• Entrez:
  • 81624(Human)
• Uniprot:
  • NP_112194(Human)