Western Blot: FOXL2 Antibody [NB100-1277] - Staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35 ug protein in RIPA buffer). FOXL2 antibody at 2 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF ...read more
Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB: Approx. 50 kDa band observed in human ovary lysates (calculated MW of 38.8 kDa band according to NP_075555.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-WhMt usage is reported in scientific literature (PMID: 24753105). ICC/IF reported in scientific literature (PMID: 27698028). IHC-Fr reported in scientific literature (PMID: 23091613). ChIP reported in scientific literature (PMID: 30212841). IHC-P reported in scientific literature (PMID: 30304016).
Read 1 Review rated 5 using NB100-1277 in the following applications:
Predicted cross-reactivity based on sequence identity: Bovine and Rat. Ferret reactivity reported in scientific literature (PMID: 25289806). Use in Ferret reported in scientific literature (PMID:25289806).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for FOXL2 Antibody
forkhead box L2
forkhead box protein L2
forkhead transcription factor FOXL2
There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Hammoud SS, Shen Y, LaRose H et al. Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration bioRxiv Jan 1 2020 (WB, Mouse)
Chaboissier MC, Richardson N, Gillot I, Gregoire EP Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of Rspo1 in mouse sex reversal bioRxiv Dec 19 2019 (IHC-P, Mouse)
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.