FOXL2 Antibody

Western Blot: FOXL2 Antibody [NB100-1277] - Staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35 ug protein in RIPA buffer). FOXL2 antibody at 2 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Ma, Rt, Bv
• Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FOXL2 Antibody Summary

• Immunogen: Peptide with sequence C-DSKTGALHSRLDL corresponding to C-Terminus according to NP_075555.1.
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
• Application Notes: WB: Approx. 50 kDa band observed in human ovary lysates (calculated MW of 38.8 kDa band according to NP_075555.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-WhMt usage is reported in scientific literature (PMID: 24753105). ICC/IF reported in scientific literature (PMID: 27698028). IHC-Fr reported in scientific literature (PMID: 23091613). ChIP reported in scientific literature (PMID: 30212841). IHC-P reported in scientific literature (PMID: 30304016).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine and Rat. Ferret reactivity reported in scientific literature (PMID: 25289806). Use in Ferret reported in scientific literature (PMID:25289806).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FOXL2 Antibody

• BPES
• BPES1
• BPES1PINTO
• forkhead box L2
• forkhead box protein L2
• forkhead transcription factor FOXL2
• FOXL2
• PFRK
• PINTO
• POF3

Background

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXL2 Antibody (NB100-1277)(41)

Publications using NB100-1277

• Zhang J, Yan L, Wang Y et al. In vivo and in vitro activation of dormant primordial follicles by EGF treatment in mouse and human Clinical and translational medicine Sep 1 2020 [PMID: 32997412] (IHC, Human)
• Li YH, Chen TM, Huang BM et al. FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture Biol. Reprod. Sep 4 2020 [PMID: 32886743] (WB)
• Richardson N, Gillot I, Gregoire EP et al. Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals Elife May 26 2020 [PMID: 32450947] (ICC/IF, Mouse)
• De Cian MC, Gregoire EP, Le Rolle M et al. R-spondin2 signaling is required for oocyte-driven intercellular communication and follicular growth Cell Death Differ. Apr 27 2020 [PMID: 32341451] (IHC, IHC-P, Mouse)
• Yamashiro C, Sasaki K, Yokobayashi S et al. Generation of human oogonia from induced pluripotent stem cells in culture Nat Protoc Apr 1 2020 [PMID: 32231324]
• FOXO1 negates the cooperative action of FOXL2C134W and SMAD3 in CYP19 expression in HGrC1 cells by sequestering SMAD3. Belli, M;Secchi, C;Stupack J Endocr Soc [PMID: 31701078] (WB, Human)
• Garcia-Moreno SA, Lin YT, Futtner CR, et al. CBX2 is required to stabilize the testis pathway by repressing Wnt signaling PLoS Genet. May 22 2019 [PMID: 31116734] (ICC/IF, Mouse)
• Fujisawa M, Moh-Moh-Aung A, Zeng Z et al. Ovarian stromal cells as a source of cancer-associated fibroblasts in human epithelial ovarian cancer: A histopathological study. PLoS ONE. Oct 10 2018 [PMID: 30304016] (IHC-P, Human)
• Nicol B, Grimm SA, Gruzdev A et al. Genome-wide identification of FOXL2 binding and characterization of FOXL2 feminizing action in the fetal gonads. Hum. Mol. Genet. Sep 13 2018 [PMID: 30212841] (ChIP, Mouse)
• Liu C, Rodriguez KF, Brown PR, Yao HH. Reproductive, physiological, and molecular outcomes in female mice deficient in Dhh and Ihh Endocrinology May 16 2018 [PMID: 29788357] (IHC, Mouse)
• Belli M, Iwata N, Nakamura T et al. FOXL2C134W-induced CYP19 expression via cooperation with SMAD3 in HGrC1 cells. Endocrinology 2018 Feb 19 [PMID: 29471425] (Mouse)
• Abe A, Ohishi Y, Miyazaki T, Ozono K et al. Microcystic pattern should be recognized as part of the morphologic spectrum of solid-pseudopapillary neoplasm of the pancreas Histopathology 2017 Aug 31 [PMID: 28858381] (Human)
• Gonen N, Quinn A, O'Neill HC et al. Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone PLoS Genet Jan 1 2017 [PMID: 28045957] (ICC/IF, Mouse)
• Ikeda Y, Tagami A, Komada M, Takahashi M. Expression of Kisspeptin in Gonadotrope Precursors in the Mouse Pituitary during Embryonic and Postnatal Development and in Adulthood. Neuroendocrinology. Nov 21 2016 [PMID: 27871073]
• Dorner J, Martinez Rodriguez V, Ziegler R et al. GLI1(+) progenitor cells in the adrenal capsule of the adult mouse give rise to heterotopic gonadal-like tissue Mol. Cell. Endocrinol. Aug 29 2016 [PMID: 27585489] (ICC/IF, IHC, Mouse)
• Spectrum WM. Juvenile Granulosa Cell Tumors of the Testis: A Clinicopathologic Study of 70 Cases With Emphasis on Its Wide Morphologic Spectrum American Journal of Surgical Pathology. 2015 Jun 05 [PMID: 26076062] (IHC-P, Human)
• Hammoud SS, Shen Y, LaRose H et al. Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration bioRxiv Jan 1 2020 (WB, Mouse)
• Chaboissier MC, Richardson N, Gillot I, Gregoire EP Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of Rspo1 in mouse sex reversal bioRxiv Dec 19 2019 (IHC-P, Mouse)
• Hikabe O, Hamazaki N, Nagamatsu G et al. Reconstitution in vitro of the entire cycle of the mouse female germ line. Nature Oct 17 2016 [PMID: 27750280] (IHC, Mouse)
• Batchvarov IS, Taylor RW, Bustamante-Marin X et al. A grafted ovarian fragment rescues host fertility after chemotherapy. Mol. Hum. Reprod. Oct 3 2016 [PMID: 27698028] (ICC/IF, Mouse)
• Arora R, Abby E, Ross AD et al. Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary. J Cell Sci. May 19 2016 [PMID: 27199373] (ICC/IF, Mouse)
• Feng L, Wang Y, Cai H et al. ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. J. Cell. Sci. 2016 Apr 15 [PMID: 27084580] (IHC-P, Mouse)
• Croce S, de Kock L, Boshari T et al. Uterine Tumor Resembling Ovarian Sex Cord Tumor (UTROSCT) Commonly Exhibits Positivity With Sex Cord Markers FOXL2 and SF-1 but Lacks FOXL2 and DICER1 Mutations. Int. J. Gynecol. Pathol. 2015 Nov 23 [PMID: 26598979] (IHC-P, Human)
• Barratt CL A grafted ovarian fragment rescues host fertility after chemotherapy Mol Hum Reprod. 2016 Oct 11 [PMID: 26590382] (ICC/IF, Mouse)
• Teng Z, Wang C, Wang Y et al. S100A8, an Oocyte-Specific Chemokine, Directs the Migration of Ovarian Somatic Cells During Mice Primordial Follicle Assembly. J. Cell. Physiol. 2015 May 06 [PMID: 25953201] (IHC-P, Mouse)
  
  Details:
  FOXL2 antibody (Imgenex IMG-3228) was used for IHC-P analysis of FOXL2 protein (as pre-granulosa cell specific marker) in tissue structures / reconstructed follicular-like cell masses which were created from murine ovarian cells. The immuno assay involved - 4% PFA fixation, 5um paraffin sections, antigen retrieval via boiling in 0.01% sodium citrate buffer, use of FOXL2 primary antibody at 1:100 dilution with 4C ON incubation, detection with IF labelled secondary (Fig. 1C).
• Schillebeeckx M, Pihlajoki M, Gretzinger E et al. Novel markers of gonadectomy-induced adrenocortical neoplasia in the mouse and ferret. Mol. Cell. Endocrinol. 2014 Oct 05 [PMID: 25289806] (IHC-P, Mouse, Ferret)
  
  Details:
  FOXL2 antibody used for IHC-P on adrenocortical tumor tissues obtained from mouse and ferrets gonadectomy-induced adrenocortical neoplasia model - 4% PFA ON fixation, paraffin embedding, 5um sections, primary used at 1:400 dilution, donkey anti-goat biotinylated IgG secondary, avidin-biotin immunoperoxidase system - DAB detection, no primary negative controls (Staining images -Fig?4, Fig?5, Fig S1).
• Arora R, Altman E, Tran ND et al. Novel domains of expression for orphan receptor tyrosine kinase Ror2 in the human and mouse reproductive system. Dev. Dyn. 2014 Apr 18 [PMID: 24753105] (IHC-WhMt, Human)
• The Expression of FOXL2 in Pancreatic, Hepatobiliary, and Renal Tumors with Ovarian-Type Stroma. Westerhoff M, Tretiakova M, Hart J et al. Hum Pathol [PMID: 24746205] (IHC-P, Human)
• Umeda S, Tateno M, Miyagi E et al. Uterine tumors resembling ovarian sex cord tumors (UTROSCT) with metastasis: clinicopathological study of two cases. Int J Clin Exp Pathol 2014 Apr 03 [PMID: 24696722] (IHC-P, Human)
  
  Details:
  Ovarian sex cord tumor: Fig 3C, Table 2.
• Anttonen M, Pihlajoki M, Andersson N et al. FOXL2, GATA4, and SMAD3 co-operatively modulate gene expression, cell viability and apoptosis in ovarian granulosa cell tumor cells. PLoS ONE. 2014 Jan 13 [PMID: 24416423] (IHC-P, WB, Human)
  
  Details:
  WB: FOXL2-transfected COS-7 and COV434 cells, Fig 1. IHC(P): Proliferating ovarian follicles (Fig 4) and granulosa cell tumors (Tables 1,2). Notes: The specificity of the FOXL2 pAb was validated in FOXL2 transfected cells by WB (Fig1). In IHC(P), FOXL2 wa
• Kommoss S, Gilks CB, Penzel R et al. A current perspective on the pathological assessment of FOXL2 in adult-type granulosa cell tumours of the ovary. Histopathology. 2014 Feb 1 [PMID: 24192202] (IHC-P, Human)
• Maillet D, Goulvent T, Rimokh R et al. Impact of a second opinion using expression and molecular analysis of FOXL2 for sex cord-stromal tumors. A study of the GINECO group & the TMRO network. Gynecol Oncol 2013 Oct 22 [PMID: 24157616] (IHC-P, Human)
• Maatouk DM, Mork L, Hinson A et al. Germ cells are not required to establish the female pathway in mouse fetal gonads PLoS One 2012 [PMID: 23091613] (IHC-Fr, ICC/IF, Mouse)
• Jameson SA, Lin YT, Capel B. Testis development requires the repression of Wnt4 by Fgf signaling Dev Biol 2012 Jun 13 [PMID: 22705479] (IHC, ICC/IF, Mouse)
• Mork L, Maatouk DM, McMahon JA et al. Temporal Differences in Granulosa Cell Specification in the Ovary Reflect Distinct Follicle Fates in Mice. Biology of Reproduction. 2011 Oct 5. [PMID: 21976597]
• Al-Agha OM, Huwait HF, Chow C et al. FOXL2 is a sensitive and specific marker for sex cord-stromal tumors of the ovary. Am J Surg Pathol. 2011 Apr [PMID: 21378549] (IHC-P)
  
  Details:
  IHC (p): Human ovarian cell tumors, Tables 1,2 and Figs 1-4. Note: The FOXL2/BPES IMG-3228 antibody was used at a dilution of 1:25 (20 ug/ml). FOXL2 protein expression was identified as a sensitive and specific marker for sex cord-stromal ovarian tumors (
• Pulinilkunnil T, He H, Kong D et al. Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem;286(11):8798-809. 2011 Mar 18. [PMID: 21209093]
• Lamba P, Wang Y, Tran S et al. Activin A regulates porcine follicle-stimulating hormone beta-subunit transcription via cooperative actions of SMADs and FOXL2. Endocrinology. 2010 Nov [PMID: 20810560] (WB, Mouse)
  
  Details:
  FOXL2/BPES (IMG-3228). WB: Immortalized murine gonadotrope (LbetaT2) cell line, Fig 2B.
• Jamieson S, Butzow R, Andersson N et al. The FOXL2 C134W mutation is characteristic of adult granulosa cell tumors of the ovary. Mod Pathol. 2010 Nov [PMID: 20693978] (Human)
  
  Details:
  IHC (p): Human ovarian granulosa cell tumors, Fig 1. Note: The FOXL2/BPES IMG-3228 antibody was used at a dilution of 1:400 (1.25 ug/ml). Both a diffuse and differentiated adult granulosa cell tumor had strong nuclear positivity of FOXL2. Another presuppo
• Blount AL, Schmidt K, Justice NJ et al. FoxL2 and Smad3 coordinately regulate follistatin gene transcription. J Biol Chem. 2009 Mar 20 [PMID: 19106105] (WB, ChIP, IHC-Fr, Mouse)
  
  Details:
  Mouse gonadotrope-derived alphaT3-1, WB: Figs. 4, 5a,b,c, 6a,b, 11a,b,d; ChIP: Fig. 5b; IHC (frozen): Fig. 3a,d,g.
• Crisponi L, Deiana M, Loi A et al. The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet 2001 Feb [PMID: 11175783]

Review for FOXL2 Antibody (NB100-1277) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Ripla Arora IF Mouse 07/17/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Ovary
• Species: Mouse

Blocking

• Blocking Details: PBS+ 0.1% Triton + 5% Donkey Serum, 20 minutes at room temperature

Primary Anitbody

• Dilution Ratio: 1:100, incubated at 4 degreeC overnight in blocking buffer

Secondary Antibody

• Secondary Description: Alexa Fluor Donkey anti-Goat

Details

• Detection Notes: Confocal microscopy
• Fixation Details: PFA fixed ovaries were section and cryosectioned and treated with citrate buffer for antigen retreival
• Wash Description: PBS

Bioinformatics

• Gene Symbol: FOXL2
• Entrez:
  • 668(Human)
  • 26927(Mouse)
• Uniprot:
  • P58012()