FOXL2 Antibody

Images

 
Western Blot: FOXL2 Antibody [NB100-1277] - Staining of Human (A) and Mouse (B) Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - (2ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). Image from verified customer ...read more

Product Details

Summary
Reactivity Hu, Mu, FtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, ICC/IF, IHC-WhMt
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FOXL2 Antibody Summary

Immunogen
Peptide with sequence C-DSKTGALHSRLDL corresponding to C-Terminus according to NP_075555.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA detection limit 1:32000
  • Immunofluorescence
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
Application Notes
WB: Approx. 50 kDa band observed in human ovary lysates (calculated MW of 38.8 kDa band according to NP_075555.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Ovary shows nuclear staining in select cells. IHC-WM usage is reported in scientific literature (PMID: 24753105).
Reviewed Applications
Read 1 Review rated 5
using
NB100-1277 in the following applications:

Publications
Read Publications using
NB100-1277 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine and Rat. Ferret reactivity reported in scientific literature (PMID:25289806).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXL2 Antibody

  • BPES
  • BPES1PINTO
  • forkhead box L2
  • forkhead box protein L2
  • forkhead transcription factor FOXL2
  • PFRK
  • POF3

Background

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOXL2 Antibody (NB100-1277)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Ferret.

We have publications tested in 7 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ChIP
(2)
ICC/IF
(6)
IHC
(4)
IHC-Fr
(2)
IHC-P
(13)
IHC-WhMt
(1)
WB
(3)
All Applications
Filter By Species
Human
(12)
Mouse
(15)
Ferret
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB100-1277 Applications Species
Fujisawa M, Moh-Moh-Aung A, Zeng Z et al. Ovarian stromal cells as a source of cancer-associated fibroblasts in human epithelial ovarian cancer: A histopathological study. PLoS ONE. Oct 10 2018 [PMID: 30304016] (IHC-P, Human) IHC-P Human
Nicol B, Grimm SA, Gruzdev A et al. Genome-wide identification of FOXL2 binding and characterization of FOXL2 feminizing action in the fetal gonads. Hum. Mol. Genet. Sep 13 2018 [PMID: 30212841] (ChIP, Mouse) ChIP Mouse
Liu C, Rodriguez KF, Brown PR, Yao HH. Reproductive, physiological, and molecular outcomes in female mice deficient in Dhh and Ihh Endocrinology May 16 2018 [PMID: 29788357] (IHC, Mouse) IHC Mouse
Belli M, Iwata N, Nakamura T et al. FOXL2C134W-induced CYP19 expression via cooperation with SMAD3 in HGrC1 cells. Endocrinology 2018 Feb 19 [PMID: 29471425] (Mouse) Mouse
Abe A, Ohishi Y, Miyazaki T, Ozono K et al. Microcystic pattern should be recognized as part of the morphologic spectrum of solid-pseudopapillary neoplasm of the pancreas Histopathology 2017 Aug 31 [PMID: 28858381] (Human) Human
Gonen N, Quinn A, O'Neill HC et al. Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone PLoS Genet Jan 1 2017 [PMID: 28045957] (ICC/IF, Mouse) ICC/IF Mouse
Ikeda Y, Tagami A, Komada M, Takahashi M. Expression of Kisspeptin in Gonadotrope Precursors in the Mouse Pituitary during Embryonic and Postnatal Development and in Adulthood. Neuroendocrinology. Nov 21 2016 [PMID: 27871073]
Hikabe O, Hamazaki N, Nagamatsu G et al. Reconstitution in vitro of the entire cycle of the mouse female germ line. Nature Oct 17 2016 [PMID: 27750280] (IHC, Mouse) IHC Mouse
Batchvarov IS, Taylor RW, Bustamante-Marin X et al. A grafted ovarian fragment rescues host fertility after chemotherapy. Mol. Hum. Reprod. Oct 3 2016 [PMID: 27698028] (ICC/IF, Mouse) ICC/IF Mouse
Dorner J, Martinez Rodriguez V, Ziegler R et al. GLI1(+) progenitor cells in the adrenal capsule of the adult mouse give rise to heterotopic gonadal-like tissue Mol. Cell. Endocrinol. Aug 29 2016 [PMID: 27585489] (ICC/IF, IHC, Mouse) ICC/IF, IHC Mouse
Show All 32 Publications.

Review for FOXL2 Antibody (NB100-1277) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1277:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence FOXL2 NB100-1277
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reviewed by:
Ripla Arora
IF Mouse 07/17/2015
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Summary

ApplicationImmunofluorescence
Sample TestedMouse Ovary
SpeciesMouse

Blocking

Blocking DetailsPBS+ 0.1% Triton + 5% Donkey Serum, 20 minutes at room temperature

Primary Anitbody

Dilution Ratio1:100, incubated at 4 degreeC overnight in blocking buffer

Secondary Antibody

Secondary DescriptionAlexa Fluor Donkey anti-Goat

Details

Detection NotesConfocal microscopy
Fixation DetailsPFA fixed ovaries were section and cryosectioned and treated with citrate buffer for antigen retreival
Wash DescriptionPBS

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FOXL2 Antibody (NB100-1277) (0)

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Secondary Antibodies

 

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Ripla Arora
07/17/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol FOXL2
Entrez
Uniprot