FOXL2 Antibody

Images

 
Western Blot: FOXL2 Antibody [NB100-1277] - Staining of Mouse Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ma, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FOXL2 Antibody Summary

Immunogen
Peptide with sequence C-DSKTGALHSRLDL corresponding to C-Terminus according to NP_075555.1.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
IHC-WhMt usage reported in scientific literature (PMID: 24753105). ICC/IF reported in scientific literature (PMID: 27698028). IHC-Fr reported in scientific literature (PMID: 23091613). ChIP reported in scientific literature (PMID: 30212841). IHC-P reported in scientific literature (PMID: 30304016).
Reviewed Applications
Read 1 Review rated 5
using
NB100-1277 in the following applications:

Publications
Read Publications using
NB100-1277 in the following applications:

Reactivity Notes

Use in Bovine, Human, Mouse reported in scientific literature (PMID: 33956944). Ferret reactivity reported in scientific literature (PMID: 25289806).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXL2 Antibody

  • BPES
  • BPES1
  • BPES1PINTO
  • forkhead box L2
  • forkhead box protein L2
  • forkhead transcription factor FOXL2
  • FOXL2
  • PFRK
  • PINTO
  • POF3

Background

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOXL2 Antibody (NB100-1277)(53)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Chicken, Ferret.

We have publications tested in 7 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ChIP
(2)
ICC/IF
(13)
IHC
(9)
IHC-Fr
(2)
IHC-P
(17)
IHC-WhMt
(1)
WB
(7)
All Applications
Filter By Species
Human
(17)
Mouse
(25)
Bovine
(1)
Chicken
(1)
Ferret
(1)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NB100-1277 Applications Species
Lowe MG Mechanisms Regulating PGC Specification and Epigenomic Reprogramming Thesis Jan 1 2022
Li Y, Overland M, Derpinghaus A et al. Development of the human fetal testis: Morphology and expression of cellular differentiation markers Differentiation Mar 1 2022 [PMID: 35490077] (IHC, Human) IHC Human
Du H, Guo Y, Wu X, Gong Y FOXL2 regulates the expression of the Col4a1 collagen gene in chicken granulosa cells Molecular reproduction and development Feb 1 2022 [PMID: 35122350] (ICC/IF) ICC/IF
Xu X, Mu L, Li L et al. Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging Science advances Jan 14 2022 [PMID: 35020427] (IHC-P, Mouse) IHC-P Mouse
Adeniran BV, Bjarkadottir BD, Appeltant R et al. Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid Human reproduction (Oxford, England) May 6 2021 [PMID: 33956944] (IHC, Bovine, Human, Mouse) IHC Bovine, Human, Mouse
Sasaki K, Oguchi A, Cheng K et al. The embryonic ontogeny of the gonadal somatic cells in mice and monkeys Cell reports May 4 2021 [PMID: 33951437]
Ikeda Y, Tagami A, Maekawa M, Nagai A The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation Scientific reports Feb 24 2021 [PMID: 33627800] (IHC-P, Mouse) IHC-P Mouse
Bunce CM Reinterpreting the Organizing Principles of Sex Determination and Gonadogenesis in the Mouse Thesis Jan 1 2021 (ICC/IF, Mouse) ICC/IF Mouse
Guo Y, Cheng L, Li X Et al. Transcriptional regulation of CYP19A1 expression in chickens: ESR1, ESR2 and NR5A2 form a functional network General and comparative endocrinology Oct 27 2021 [PMID: 34710471] (WB, ICC/IF, IHC, Chicken) WB, ICC/IF, IHC Chicken
Ruthig VA, Yokonishi T, Friedersdorf MB, et al. A transgenic DND1GFP fusion allele reports in vivo expression and RNA binding targets in undifferentiated mouse germ cells Biology of reproduction Jan 4 2021 [PMID: 33394034]
Show All 53 Publications.

Review for FOXL2 Antibody (NB100-1277) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1277:
Filter by Applications
Immunofluorescence
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence FOXL2 NB100-1277
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence Mouse 07/17/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Ovary
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FOXL2 Antibody (NB100-1277) (0)

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Secondary Antibodies

 

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Recent Reviews

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Verified Customer
07/17/2015
Application: Immunofluorescence
Species: Mouse

Bioinformatics

Gene Symbol FOXL2
Entrez
Uniprot