FOXL2 Antibody

Images

 
Western Blot: FOXL2 Antibody [NB100-1277] - Staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35 ug protein in RIPA buffer). FOXL2 antibody at 2 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF ...read more
Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Ma, Rt, BvSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FOXL2 Antibody Summary

Immunogen
Peptide with sequence C-DSKTGALHSRLDL corresponding to C-Terminus according to NP_075555.1.
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
Application Notes
WB: Approx. 50 kDa band observed in human ovary lysates (calculated MW of 38.8 kDa band according to NP_075555.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-WhMt usage is reported in scientific literature (PMID: 24753105). ICC/IF reported in scientific literature (PMID: 27698028). IHC-Fr reported in scientific literature (PMID: 23091613). ChIP reported in scientific literature (PMID: 30212841). IHC-P reported in scientific literature (PMID: 30304016).
Reviewed Applications
Read 1 Review rated 5
using
NB100-1277 in the following applications:

Publications
Read Publications using
NB100-1277 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine and Rat. Ferret reactivity reported in scientific literature (PMID: 25289806). Use in Ferret reported in scientific literature (PMID:25289806).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXL2 Antibody

  • BPES
  • BPES1
  • BPES1PINTO
  • forkhead box L2
  • forkhead box protein L2
  • forkhead transcription factor FOXL2
  • FOXL2
  • PFRK
  • PINTO
  • POF3

Background

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1596
Species: Hu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-52823
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF3075
Species: Hu
Applications: WB, Simple Western, ICC
NBP2-01770
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85217
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84071
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-37463
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
AF475
Species: Mu
Applications: WB, IHC
NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-80104
Species: Hu
Applications: WB
NBP1-83192
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PP-H7431-00
Species: Hu
Applications: WB
H00007536-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
NBP2-80459
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NBP1-85368
Species: Hu, RM
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01151
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-1277
Species: Hu, Mu, Ma, Rt, Bv
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt

Publications for FOXL2 Antibody (NB100-1277)(41)

We have publications tested in 3 confirmed species: Human, Mouse, Ferret.

We have publications tested in 7 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ChIP
(2)
ICC/IF
(9)
IHC
(6)
IHC-Fr
(2)
IHC-P
(14)
IHC-WhMt
(1)
WB
(6)
All Applications
Filter By Species
Human
(13)
Mouse
(21)
Ferret
(1)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NB100-1277 Applications Species
Zhang J, Yan L, Wang Y et al. In vivo and in vitro activation of dormant primordial follicles by EGF treatment in mouse and human Clinical and translational medicine Sep 1 2020 [PMID: 32997412] (IHC, Human) IHC Human
Li YH, Chen TM, Huang BM et al. FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture Biol. Reprod. Sep 4 2020 [PMID: 32886743] (WB) WB
Richardson N, Gillot I, Gregoire EP et al. Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals Elife May 26 2020 [PMID: 32450947] (ICC/IF, Mouse) ICC/IF Mouse
De Cian MC, Gregoire EP, Le Rolle M et al. R-spondin2 signaling is required for oocyte-driven intercellular communication and follicular growth Cell Death Differ. Apr 27 2020 [PMID: 32341451] (IHC, IHC-P, Mouse) IHC, IHC-P Mouse
Yamashiro C, Sasaki K, Yokobayashi S et al. Generation of human oogonia from induced pluripotent stem cells in culture Nat Protoc Apr 1 2020 [PMID: 32231324]
FOXO1 negates the cooperative action of FOXL2C134W and SMAD3 in CYP19 expression in HGrC1 cells by sequestering SMAD3. Belli, M;Secchi, C;Stupack J Endocr Soc [PMID: 31701078] (WB, Human) WB Human
Garcia-Moreno SA, Lin YT, Futtner CR, et al. CBX2 is required to stabilize the testis pathway by repressing Wnt signaling PLoS Genet. May 22 2019 [PMID: 31116734] (ICC/IF, Mouse) ICC/IF Mouse
Fujisawa M, Moh-Moh-Aung A, Zeng Z et al. Ovarian stromal cells as a source of cancer-associated fibroblasts in human epithelial ovarian cancer: A histopathological study. PLoS ONE. Oct 10 2018 [PMID: 30304016] (IHC-P, Human) IHC-P Human
Nicol B, Grimm SA, Gruzdev A et al. Genome-wide identification of FOXL2 binding and characterization of FOXL2 feminizing action in the fetal gonads. Hum. Mol. Genet. Sep 13 2018 [PMID: 30212841] (ChIP, Mouse) ChIP Mouse
Liu C, Rodriguez KF, Brown PR, Yao HH. Reproductive, physiological, and molecular outcomes in female mice deficient in Dhh and Ihh Endocrinology May 16 2018 [PMID: 29788357] (IHC, Mouse) IHC Mouse
Show All 41 Publications.

Review for FOXL2 Antibody (NB100-1277) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1277:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence FOXL2 NB100-1277
Enlarge
5
reviewed by:
Ripla Arora
IF Mouse 07/17/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Ovary
SpeciesMouse

Blocking

Blocking DetailsPBS+ 0.1% Triton + 5% Donkey Serum, 20 minutes at room temperature

Primary Anitbody

Dilution Ratio1:100, incubated at 4 degreeC overnight in blocking buffer

Secondary Antibody

Secondary DescriptionAlexa Fluor Donkey anti-Goat

Details

Detection NotesConfocal microscopy
Fixation DetailsPFA fixed ovaries were section and cryosectioned and treated with citrate buffer for antigen retreival
Wash DescriptionPBS

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FOXL2 Antibody (NB100-1277) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional FOXL2 Products

Bioinformatics Tool for FOXL2 Antibody (NB100-1277)

Discover related pathways, diseases and genes to FOXL2 Antibody (NB100-1277). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FOXL2 Antibody (NB100-1277)

Discover more about diseases related to FOXL2 Antibody (NB100-1277).
 

Pathways for FOXL2 Antibody (NB100-1277)

View related products by pathway.

PTMs for FOXL2 Antibody (NB100-1277)

Learn more about PTMs related to FOXL2 Antibody (NB100-1277).

Blogs on FOXL2

There are no specific blogs for FOXL2, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ripla Arora
07/17/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol FOXL2
Entrez
Uniprot