FOXL2 Antibody

Western Blot: FOXL2 Antibody [NB100-1277] - (1ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - (8ug/ml) staining of paraffin embedded Human Ovary. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.

• Reactivity: Hu, Mu, Bv, Ma, Rt
• Applications: WB, ELISA, ICC/IF, IHC, ChIP, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FOXL2 Antibody Summary

• Immunogen: Peptide with sequence C-DSKTGALHSRLDL corresponding to C-Terminus according to NP_075555.1.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
  • Immunohistochemistry 8 ug/mL
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx. 50kDa band observed in Mouse Ovary lysates and in preliminary testing of Human Uterus and Rat Ovary lysate (calculated MW of 38.9kDa according to Mouse NP_036150.1). This molecular weight is routinely observed by other sources. Primary incubation 1 hour at room temperature.
  
  IHC-WhMt usage reported in scientific literature (PMID: 24753105). ICC/IF reported in scientific literature (PMID: 27698028). IHC-Fr reported in scientific literature (PMID: 23091613). ChIP reported in scientific literature (PMID: 30212841). IHC-P reported in scientific literature (PMID: 30304016).

Reactivity Notes

Use in Bovine, Human, Mouse reported in scientific literature (PMID: 33956944). Ferret reactivity reported in scientific literature (PMID: 25289806).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FOXL2 Antibody

• BPES
• BPES1
• BPES1PINTO
• forkhead box L2
• forkhead box protein L2
• forkhead transcription factor FOXL2
• FOXL2
• PFRK
• PINTO
• POF3

Background

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXL2 Antibody (NB100-1277)(67)

Publications using NB100-1277

• Danti L, Lundin K, Sepponen K et al. CRISPR/Cas9-mediated activation of NR5A1 steers female human embryonic stem cell-derived bipotential gonadal-like cells towards a steroidogenic cell fate J Ovarian Res 2023-09-20 [PMID: 37726790]
• Lim J, Lee HL, Nguyen J et al. Adolescent exposure to low-dose ?9-tetrahydrocannabinol (THC) depletes the ovarian reserve in female mice Toxicological sciences : an official journal of the Society of Toxicology 2023-03-13 [PMID: 36912754] (IHC-Fr, Mouse)
  
  Details:
  1:200 dilution
• Pierson Smela MD, Kramme CC, Fortuna PRJ et al. Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression eLife 2023-02-21 [PMID: 36803359] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC/IF 1:250
• Yu X, Wang N, Zhang Y et al. Oocyte Arrested at Metaphase II Stage Were Derived From Human Pluripotent Stem Cells in Vitro Research Square 2021-03-17 [PMID: 36735215] (ICC/IF, Human)
• Cheng Y, Zhang Z, Zhang G et al. The Male-Biased Expression of miR-2954 Is Involved in the Male Pathway of Chicken Sex Differentiation Cells 2022-12-20 [PMID: 36611798] (IHC-Fr, Chicken)
• Wang Y, Zhang J, Liang J et al. In vivo promotion of primordial follicle activation by stem cell factor treatment in mice with premature ovarian insufficiency and advanced age Molecular human reproduction 2022-12-07 [PMID: 36477300]
• Zhang S, Mu L, Wang H et al. Quantitative proteomic analysis uncovers protein-expression profiles during gonadotropin-dependent folliculogenesis in mice Biology of reproduction 2022-12-08 [PMID: 36477298]
• Lowe MG Mechanisms Regulating PGC Specification and Epigenomic Reprogramming Thesis 2022-01-01
• Bridges K, Yao HH, Nicol B Loss of Runx1 Induces Granulosa Cell Defects and Development of Ovarian Tumors in the Mouse International journal of molecular sciences 2022-11-21 [PMID: 36430923] (ICC/IF, Mouse)
• Overland M, Li Y, Derpinghaus A et al. Development of the human ovary: Fetal through pubertal ovarian morphology, folliculogenesis and expression of cellular differentiation markers Differentiation 2022-10-01 [PMID: 36347737] (IF/IHC, Human)
• Thomson E, Tran M, Robevska G et al. Functional genomics analysis identifies loss of HNF1B function as a cause of Mayer-Rokitansky-K�ster-Hauser syndrome Human Molecular Genetics 2023-03-06 [PMID: 36282544] (IHC, ICC/IF)
• Del Valle JS, Mancini V, Laverde Garay M et al. Dynamic in vitro culture of cryopreserved-thawed human ovarian cortical tissue using a microfluidics platform does not improve early folliculogenesis Front Endocrinol (Lausanne) 2022-07-29 [PMID: 35966050]
• Yu J, Xie X, Ma Y et al. Effects and potential mechanism of Ca2+/calmodulin dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle International journal of molecular medicine 2022-10-01 [PMID: 35929517] (IHC-P, Mouse)
• Mizuta K, Katou Y, Nakakita B et al. Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys The EMBO journal 2022-08-01 [PMID: 35912849]
• Rodriguez KF, Brown PR, Amato CM et al. Somatic cell fate maintenance in mouse fetal testes via autocrine/paracrine action of AMH and activin B Nature communications 2022-07-15 [PMID: 35840551] (IHC-P, Mouse)
• Dai Y, Bo Y, Wang P et al. Asynchronous embryonic germ cell development leads to a heterogeneity of postnatal ovarian follicle activation and may influence the timing of puberty onset in mice BMC Biology 2022-12-01 [PMID: 35550124]
• Li Y, Overland M, Derpinghaus A et al. Development of the human fetal testis: Morphology and expression of cellular differentiation markers Differentiation 2022-03-01 [PMID: 35490077] (IF/IHC, Human)
• Du H, Guo Y, Wu X, Gong Y FOXL2 regulates the expression of the Col4a1 collagen gene in chicken granulosa cells Molecular reproduction and development 2022-02-01 [PMID: 35122350] (ICC/IF)
• Xu X, Mu L, Li L et al. Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging Science advances 2022-01-14 [PMID: 35020427] (IHC-P, Mouse)
• Guo Y, Cheng L, Li X Et al. Transcriptional regulation of CYP19A1 expression in chickens: ESR1, ESR2 and NR5A2 form a functional network General and comparative endocrinology 2021-10-27 [PMID: 34710471] (WB, IF/IHC, ICC/IF, Chicken)
• Hammoud SS, Shen Y, LaRose H et al. Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration Nat Commun 2021-06-24 [PMID: 34162856]
• Adeniran BV, Bjarkadottir BD, Appeltant R et al. Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid Human reproduction (Oxford, England) 2021-05-06 [PMID: 33956944] (IF/IHC, Bovine, Human, Mouse)
• Sasaki K, Oguchi A, Cheng K et al. The embryonic ontogeny of the gonadal somatic cells in mice and monkeys Cell reports 2021-05-04 [PMID: 33951437]
• Ikeda Y, Tagami A, Maekawa M, Nagai A The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation Scientific reports 2021-02-24 [PMID: 33627800] (IHC-P, Mouse)
• Ruthig VA, Yokonishi T, Friedersdorf MB, et al. A transgenic DND1GFP fusion allele reports in vivo expression and RNA binding targets in undifferentiated mouse germ cells Biology of reproduction 2021-01-04 [PMID: 33394034]
• Zhang J, Yan L, Wang Y et al. In vivo and in vitro activation of dormant primordial follicles by EGF treatment in mouse and human Clinical and translational medicine 2020-09-01 [PMID: 32997412] (IF/IHC, Human)
• Nicol B, Rodriguez K, Yao HH. Aberrant and constitutive expression of FOXL2 impairs ovarian development and functions in mice Biology of Reproduction 2020-10-29 [PMID: 32945847] (IHC-Fr, ICC/IF)
• Li YH, Chen TM, Huang BM et al. FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture Biol. Reprod. 2020-09-04 [PMID: 32886743] (WB)
• Richardson N, Gillot I, Gregoire EP et al. Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals Elife 2020-05-26 [PMID: 32450947] (ICC/IF, Mouse)
• De Cian MC, Gregoire EP, Le Rolle M et al. R-spondin2 signaling is required for oocyte-driven intercellular communication and follicular growth Cell Death Differ. 2020-04-27 [PMID: 32341451] (IHC-P, IF/IHC, Mouse)
• Yamashiro C, Sasaki K, Yokobayashi S et al. Generation of human oogonia from induced pluripotent stem cells in culture Nat Protoc 2020-04-01 [PMID: 32231324]
• FOXO1 negates the cooperative action of FOXL2C134W and SMAD3 in CYP19 expression in HGrC1 cells by sequestering SMAD3. Belli, M;Secchi, C;Stupack J Endocr Soc [PMID: 31701078] (WB, Human)
• Garcia-Moreno SA, Lin YT, Futtner CR, et al. CBX2 is required to stabilize the testis pathway by repressing Wnt signaling PLoS Genet. 2019-05-22 [PMID: 31116734] (ICC/IF, Mouse)
• Fujisawa M, Moh-Moh-Aung A, Zeng Z et al. Ovarian stromal cells as a source of cancer-associated fibroblasts in human epithelial ovarian cancer: A histopathological study. PLoS ONE. 2018-10-10 [PMID: 30304016] (IHC-P, Human)
• Nicol B, Grimm SA, Gruzdev A et al. Genome-wide identification of FOXL2 binding and characterization of FOXL2 feminizing action in the fetal gonads. Hum. Mol. Genet. 2018-09-13 [PMID: 30212841] (Chemotaxis, Mouse)
• Liu C, Rodriguez KF, Brown PR, Yao HH. Reproductive, physiological, and molecular outcomes in female mice deficient in Dhh and Ihh Endocrinology 2018-05-16 [PMID: 29788357] (IF/IHC, Mouse)
• Belli M, Iwata N, Nakamura T et al. FOXL2C134W-induced CYP19 expression via cooperation with SMAD3 in HGrC1 cells. Endocrinology 2018-02-19 [PMID: 29471425] (Mouse)
• Abe A, Ohishi Y, Miyazaki T, Ozono K et al. Microcystic pattern should be recognized as part of the morphologic spectrum of solid-pseudopapillary neoplasm of the pancreas Histopathology 2017-08-31 [PMID: 28858381] (Human)
• Gonen N, Quinn A, O'Neill HC et al. Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone PLoS Genet 2017-01-01 [PMID: 28045957] (ICC/IF, Mouse)
• Ikeda Y, Tagami A, Komada M, Takahashi M. Expression of Kisspeptin in Gonadotrope Precursors in the Mouse Pituitary during Embryonic and Postnatal Development and in Adulthood. Neuroendocrinology. 2016-11-21 [PMID: 27871073]
• Hikabe O, Hamazaki N, Nagamatsu G et al. Reconstitution in vitro of the entire cycle of the mouse female germ line. Nature 2016-10-17 [PMID: 27750280] (IF/IHC, Mouse)
• Batchvarov IS, Taylor RW, Bustamante-Marin X et al. A grafted ovarian fragment rescues host fertility after chemotherapy. Mol. Hum. Reprod. 2016-10-03 [PMID: 27698028] (ICC/IF, Mouse)
• Dorner J, Martinez Rodriguez V, Ziegler R et al. GLI1(+) progenitor cells in the adrenal capsule of the adult mouse give rise to heterotopic gonadal-like tissue Mol. Cell. Endocrinol. 2016-08-29 [PMID: 27585489] (IF/IHC, ICC/IF, Mouse)
• Arora R, Abby E, Ross AD et al. Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary. J Cell Sci. 2016-05-19 [PMID: 27199373] (ICC/IF, Mouse)
• Feng L, Wang Y, Cai H et al. ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. J. Cell. Sci. 2016-04-15 [PMID: 27084580] (IHC-P, Mouse)
• Croce S, de Kock L, Boshari T et al. Uterine Tumor Resembling Ovarian Sex Cord Tumor (UTROSCT) Commonly Exhibits Positivity With Sex Cord Markers FOXL2 and SF-1 but Lacks FOXL2 and DICER1 Mutations. Int. J. Gynecol. Pathol. 2015-11-23 [PMID: 26598979] (IHC-P, Human)
• Barratt CL A grafted ovarian fragment rescues host fertility after chemotherapy Mol Hum Reprod. 2016-10-11 [PMID: 26590382] (ICC/IF, Mouse)
• Spectrum WM. Juvenile Granulosa Cell Tumors of the Testis: A Clinicopathologic Study of 70 Cases With Emphasis on Its Wide Morphologic Spectrum American Journal of Surgical Pathology. 2015-06-05 [PMID: 26076062] (IHC-P, Human)
• Teng Z, Wang C, Wang Y et al. S100A8, an Oocyte-Specific Chemokine, Directs the Migration of Ovarian Somatic Cells During Mice Primordial Follicle Assembly. J. Cell. Physiol. 2015-05-06 [PMID: 25953201] (IHC-P, Mouse)
  
  Details:
  FOXL2 antibody (Imgenex IMG-3228) was used for IHC-P analysis of FOXL2 protein (as pre-granulosa cell specific marker) in tissue structures / reconstructed follicular-like cell masses which were created from murine ovarian cells. The immuno assay involved - 4% PFA fixation, 5um paraffin sections, antigen retrieval via boiling in 0.01% sodium citrate buffer, use of FOXL2 primary antibody at 1:100 dilution with 4C ON incubation, detection with IF labelled secondary (Fig. 1C).
• Schillebeeckx M, Pihlajoki M, Gretzinger E et al. Novel markers of gonadectomy-induced adrenocortical neoplasia in the mouse and ferret. Mol. Cell. Endocrinol. 2014-10-05 [PMID: 25289806] (IHC-P, Ferret, Mouse)
  
  Details:
  FOXL2 antibody used for IHC-P on adrenocortical tumor tissues obtained from mouse and ferrets gonadectomy-induced adrenocortical neoplasia model - 4% PFA ON fixation, paraffin embedding, 5um sections, primary used at 1:400 dilution, donkey anti-goat biotinylated IgG secondary, avidin-biotin immunoperoxidase system - DAB detection, no primary negative controls (Staining images -Fig?4, Fig?5, Fig S1).
• Kao C S, Ulbright T M et al. Gonadoblastoma: an immunohistochemical study and comparison to Sertoli cell nodule with intratubular germ cell neoplasia, with pathogenetic implications. Histopathology 2014-01-12 [PMID: 24766183] (IHC-P, Human)
• Arora R, Altman E, Tran ND et al. Novel domains of expression for orphan receptor tyrosine kinase Ror2 in the human and mouse reproductive system. Dev. Dyn. 2014-04-18 [PMID: 24753105] (IHC-WhMt, Human)
• The Expression of FOXL2 in Pancreatic, Hepatobiliary, and Renal Tumors with Ovarian-Type Stroma. Westerhoff M, Tretiakova M, Hart J et al. Hum Pathol [PMID: 24746205] (IHC-P, Human)
• Umeda S, Tateno M, Miyagi E et al. Uterine tumors resembling ovarian sex cord tumors (UTROSCT) with metastasis: clinicopathological study of two cases. Int J Clin Exp Pathol 2014-04-03 [PMID: 24696722] (IHC-P, Human)
  
  Details:
  Ovarian sex cord tumor: Fig 3C, Table 2.
• Anttonen M, Pihlajoki M, Andersson N et al. FOXL2, GATA4, and SMAD3 co-operatively modulate gene expression, cell viability and apoptosis in ovarian granulosa cell tumor cells. PLoS ONE. 2014-01-13 [PMID: 24416423] (WB, IHC-P, Human)
  
  Details:
  WB: FOXL2-transfected COS-7 and COV434 cells, Fig 1. IHC(P): Proliferating ovarian follicles (Fig 4) and granulosa cell tumors (Tables 1,2). Notes: The specificity of the FOXL2 pAb was validated in FOXL2 transfected cells by WB (Fig1). In IHC(P), FOXL2 wa
• Kommoss S, Gilks CB, Penzel R et al. A current perspective on the pathological assessment of FOXL2 in adult-type granulosa cell tumours of the ovary. Histopathology. 2014-02-01 [PMID: 24192202] (IHC-P, Human)
• Bunce CM Reinterpreting the Organizing Principles of Sex Determination and Gonadogenesis in the Mouse Thesis 2021-01-01 (ICC/IF, Mouse)
• Maillet D, Goulvent T, Rimokh R et al. Impact of a second opinion using expression and molecular analysis of FOXL2 for sex cord-stromal tumors. A study of the GINECO group & the TMRO network. Gynecol Oncol 2013-10-22 [PMID: 24157616] (IHC-P, Human)
• Maatouk DM, Mork L, Hinson A et al. Germ cells are not required to establish the female pathway in mouse fetal gonads PLoS One 2012-01-01 [PMID: 23091613] (IHC-Fr, ICC/IF, Mouse)
• Jameson SA, Lin YT, Capel B. Testis development requires the repression of Wnt4 by Fgf signaling Dev Biol 2012-06-13 [PMID: 22705479] (ICC/IF, IF/IHC, Mouse)
• Mork L, Maatouk DM, McMahon JA et al. Temporal Differences in Granulosa Cell Specification in the Ovary Reflect Distinct Follicle Fates in Mice. Biology of Reproduction. 2011-10-05 [PMID: 21976597]
• Al-Agha OM, Huwait HF, Chow C et al. FOXL2 is a sensitive and specific marker for sex cord-stromal tumors of the ovary. Am J Surg Pathol. 2011-04-01 [PMID: 21378549] (IHC-P)
  
  Details:
  IHC (p): Human ovarian cell tumors, Tables 1,2 and Figs 1-4. Note: The FOXL2/BPES IMG-3228 antibody was used at a dilution of 1:25 (20 ug/ml). FOXL2 protein expression was identified as a sensitive and specific marker for sex cord-stromal ovarian tumors (
• Pulinilkunnil T, He H, Kong D et al. Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem. 2011-03-18 [PMID: 21209093]
  
  Details:
  WB: GAPDH Loading Control (IMG-3073). Mouse interscapular brown adipose cells, Fig 3A.
• Lamba P, Wang Y, Tran S et al. Activin A regulates porcine follicle-stimulating hormone beta-subunit transcription via cooperative actions of SMADs and FOXL2. Endocrinology. 2010-11-01 [PMID: 20810560] (WB, Mouse)
  
  Details:
  FOXL2/BPES (IMG-3228). WB: Immortalized murine gonadotrope (LbetaT2) cell line, Fig 2B.
• Jamieson S, Butzow R, Andersson N et al. The FOXL2 C134W mutation is characteristic of adult granulosa cell tumors of the ovary. Mod Pathol. 2010-11-01 [PMID: 20693978] (Human)
  
  Details:
  IHC (p): Human ovarian granulosa cell tumors, Fig 1. Note: The FOXL2/BPES IMG-3228 antibody was used at a dilution of 1:400 (1.25 ug/ml). Both a diffuse and differentiated adult granulosa cell tumor had strong nuclear positivity of FOXL2. Another presuppo
• Blount AL, Schmidt K, Justice NJ et al. FoxL2 and Smad3 coordinately regulate follistatin gene transcription. J Biol Chem. 2009-03-20 [PMID: 19106105] (IHC-Fr, WB, Chemotaxis, Mouse)
  
  Details:
  Mouse gonadotrope-derived alphaT3-1, WB: Figs. 4, 5a,b,c, 6a,b, 11a,b,d; ChIP: Fig. 5b; IHC (frozen): Fig. 3a,d,g.
• Crisponi L, Deiana M, Loi A et al. The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet 2001-02-01 [PMID: 11175783]

Review for FOXL2 Antibody (NB100-1277) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Ripla Arora IF Mouse 07/17/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Ovary
• Species: Mouse

Bioinformatics

• Gene Symbol: FOXL2
• Entrez:
  • 668(Human)
  • 26927(Mouse)
• Uniprot:
  • P58012()