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Western Blot: FOXL2 Antibody [NB100-1277] - Staining of Human (A) and Mouse (B) Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - (2ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). Image from verified customer ...read more
WB: Approx. 50 kDa band observed in human ovary lysates (calculated MW of 38.8 kDa band according to NP_075555.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Ovary shows nuclear staining in select cells. IHC-WM usage is reported in scientific literature (PMID: 24753105).
Reviewed Applications
Read 1 Review rated 5 using NB100-1277 in the following applications:
Predicted cross-reactivity based on sequence identity: Bovine and Rat. Ferret reactivity reported in scientific literature (PMID:25289806).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for FOXL2 Antibody
BPES
BPES1PINTO
forkhead box L2
forkhead box protein L2
forkhead transcription factor FOXL2
PFRK
POF3
Background
There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Westerhoff M, Tretiakova M, Hart J et al. The Expression of FOXL2 in Pancreatic, Hepatobiliary, and Renal Tumors with Ovarian-Type Stroma. Human Pathology. 2014 Feb 1 (IHC-P, Human)
Details: Normal ovarian stroma, ovarian tumors, and other tumor types with ovarian-type stroma, Tables 1,2; Fig D-F.
Bioinformatics Tool for FOXL2 Antibody (NB100-1277)
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Diseases for FOXL2 Antibody (NB100-1277)
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