DIAPH-2 Antibody (CL1113) - Azide and BSA Free

Staining of human kidney shows moderate positivity in renal glomeruli.

Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.

Staining of human pancreas shows absence of immunoreactivity (negative control).

Staining of human placenta shows strong staining in the trophoblast.

Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.

Staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DIAPH-2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL1113
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

DIAPH-2 Antibody (CL1113) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DIAPH-2 Antibody (CL1113) - Azide and BSA Free (NBP2-52967) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O60879, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DIAPH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for DIAPH-2 Antibody (CL1113) - Azide and BSA Free

• DIA2
• DIAdiaphanous (Drosophila, homolog) 2
• DIAPH2
• DIAPH-2
• diaphanous homolog 2 (Drosophila)
• Diaphanous-related formin-2
• diaphorase-2
• DRF2
• POF
• POF2
• POF2FLJ11167
• protein diaphanous homolog 2

Background

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIAPH2