DEP-1/CD148 Antibody (143-41) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human DEP-1/CD148 PE-conjugated Monoclonal Antibody (Catalog # FAB1934P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 143-41
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

DEP-1/CD148 Antibody (143-41) [Phycoerythrin] Summary

• Immunogen: Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
• Specificity: Detects human DEP-1/CD148 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRJ
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEP-1/CD148 Antibody (143-41) [Phycoerythrin]

• CD148 antigen
• CD148
• Density-enhanced phosphatase 1
• DEP1
• DEP-1
• DEP1EC 3.1.3.48
• HPTP eta
• HPTPeta
• HPTP-eta
• human density enhanced phosphatase-1
• protein tyrosine phosphatase, receptor type, J polypeptide
• protein tyrosine phosphatase, receptor type, J
• Protein-tyrosine phosphatase eta
• Protein-tyrosine phosphatase receptor type J
• PTPRJ
• receptor-type tyrosine-protein phosphatase eta
• R-PTP-ETA
• R-PTP-J
• SCC1
• susceptibility to colon cancer 1, mouse, homolog of

Background

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP Receptor Type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-Catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
2. Sorby, M. et al. (2001) Oncogene 20:5219.
3. Jandt, E. et al. (2003) Oncogene 22:4175.
4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEP-1/CD148 Antibody (FAB1934P)(3)

Publications using FAB1934P

• Magri G, Comerma L, Pybus M et al. Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals Immunity 7/16/2017 [PMID: 28709802]
• Stepanek O, Kalina T, Draber P Regulation of Src Family Kinases Involved in T Cell Receptor Signaling by Protein-tyrosine Phosphatase CD148. J. Biol. Chem., 2011-05-04;286(25):22101-12. 2011-05-04 [PMID: 21543337] (Flow Cytometry, Human)
• Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 2010-10-15;134(2):113-21. 2010-10-15 [PMID: 20951740] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PTPRJ