DEP-1/CD148 Antibody (143-41) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 143-41
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

DEP-1/CD148 Antibody (143-41) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human DEP-1/CD148 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEP-1/CD148 Antibody (143-41) [Alexa Fluor™ Plus 647]

• CD148 antigen
• CD148
• Density-enhanced phosphatase 1
• DEP1
• DEP-1
• DEP1EC 3.1.3.48
• HPTP eta
• HPTPeta
• HPTP-eta
• human density enhanced phosphatase-1
• protein tyrosine phosphatase, receptor type, J polypeptide
• protein tyrosine phosphatase, receptor type, J
• Protein-tyrosine phosphatase eta
• Protein-tyrosine phosphatase receptor type J
• PTPRJ
• receptor-type tyrosine-protein phosphatase eta
• R-PTP-ETA
• R-PTP-J
• SCC1
• susceptibility to colon cancer 1, mouse, homolog of

Background

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
2. Sorby, M. et al. (2001) Oncogene 20:5219.
3. Jandt, E. et al. (2003) Oncogene 22:4175.
4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.