Dectin-1/CLEC7A Antibody

Western Blot: Dectin-1/CLEC7A Antibody [NBP1-45514] - Analysis of Dectin-1 expression in lysate from rat kidney (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3 & 4).

Product Discontinued

Dectin-1/CLEC7A Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab286157)
• Immunogen: Synthetic peptide surrounding amino acid 161 of human Dectin-1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLEC7A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 27.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 30% glycerol, 0.5% BSA
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Dectin-1/CLEC7A Antibody

• Beta-glucan receptor
• BGR
• CD369
• CLEC7A
• CLECSF12
• CLECSF12DC-associated C-type lectin 1
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
• C-type lectin domain family 7 member A
• C-type lectin domain family 7, member A
• C-type lectin superfamily member 12
• Dectin1
• Dectin-1
• DECTIN1CANDF4
• Dendritic cell-associated C-type lectin 1
• dendritic cell-associated C-type lectin-1
• hDectin-1
• lectin-like receptor 1

Background

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Dectin-1/CLEC7A Antibody (NBP1-45514)(4)

Publications using NBP1-45514

• Iannucci J, Sen A, Grammas P Isoform-Specific Effects of Apolipoprotein E on Markers of Inflammation and Toxicity in Brain Glia and Neuronal Cells In Vitro Current issues in molecular biology 2021-05-27 [PMID: 34071762] (WB, Human)
• Iannucci J, Rao HV, Grammas P High Glucose and Hypoxia-Mediated Damage to Human Brain Microvessel Endothelial Cells Induces an Altered, Pro-Inflammatory Phenotype in BV-2 Microglia In Vitro Elife 2020-10-19 [PMID: 33136275]
• Casieri V, Matteucci M, Cavallini C, Torti M et al. Long-term Intake of Pasta Containing Barley (1-3)Beta-D-Glucan Increases Neovascularization-mediated Cardioprotection through Endothelial Upregulation of Vascular Endothelial Growth Factor and Parkin Sci Rep 2017-10-18 [PMID: 29044182] (WB, Mouse)
• Cohen-Kedar S, Baram L, Elad H et al. Human intestinal epithelial cells respond to b-glucans via Dectin-1 and Syk. Eur J Immunol. 2014-09-24 [PMID: 25251945]

Reviews for Dectin-1/CLEC7A Antibody (NBP1-45514) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 review tested in 1 species: Human.

reviewed by: silvia agostini IF 07/07/2014

Summary

• Application: Immunofluorescence

reviewed by: silvia agostini WB Human 06/30/2014

Summary

• Application: Western Blot
• Sample Tested: human endothelial cell lysate (HMEC-1 and HUVEC)
• Species: Human

FAQs for Dectin-1/CLEC7A Antibody (NBP1-45514). (Showing 1 - 1 of 1 FAQs).

1. I am looking for a human dectin-1 and 2 for western blot. How can I know if it works? Do you have any references from the literature that confirm your products? If yes-I'll be happy to read few articles who used your dectin-1 and it worked.
   • Unfortunately, to the best of our knowledge, our dectin antibodies have not been cited in literature yet, and for the peace of your mind, my suggestion to you would be to make your decision based upon the validation images shown on datasheets. You may find our Dectin 1 and Dectin 2 antibodies suitable for WB application using these links. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products. Please let me know if I can help you with any other queries.

Bioinformatics

• Gene Symbol: CLEC7A
• Entrez:
  • 64581(Human)
• Uniprot:
  • Q9BXN2(Human)