Dectin-1/CLEC7A Antibody

Images

 
Western Blot: Dectin-1/CLEC7A Antibody [NBP1-45514] - Analysis of Dectin-1 expression in lysate from rat kidney (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3 & 4).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dectin-1/CLEC7A Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 161 of human Dectin-1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLEC7A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes 28 kDa of Dectin-1 from samples of human origins. A ~16 kDa monomeric form and a 43 kDa glycoprotein can also be detected. Reactivity to other species has not been tested.
Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-45514 in the following application:

Publications
Read Publication using NBP1-45514.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dectin-1/CLEC7A Antibody

  • Beta-glucan receptor
  • BGR
  • CD369
  • CLEC7A
  • CLECSF12
  • CLECSF12DC-associated C-type lectin 1
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
  • C-type lectin domain family 7 member A
  • C-type lectin domain family 7, member A
  • C-type lectin superfamily member 12
  • Dectin1
  • Dectin-1
  • DECTIN1CANDF4
  • Dendritic cell-associated C-type lectin 1
  • dendritic cell-associated C-type lectin-1
  • hDectin-1
  • lectin-like receptor 1

Background

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dectin-1/CLEC7A Antibody (NBP1-45514)(1)

Reviews for Dectin-1/CLEC7A Antibody (NBP1-45514) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Human.
We have 2 reviews tested in 2 application: IF, WB.

Reviews using NBP1-45514:
Filter by Applications
IF
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Dectin-1/CLEC7A NBP1-45514
Enlarge
reviewed by:
silvia agostini
IF 07/07/2014
View

Summary

ApplicationImmunofluorescence
Western Blot Dectin-1/CLEC7A NBP1-45514
Enlarge
reviewed by:
silvia agostini
WB Human 06/30/2014
View

Summary

ApplicationWestern Blot
Sample Testedhuman endothelial cell lysate (HMEC-1 and HUVEC)
SpeciesHuman

Blocking

Blocking DetailsTBS-T 3% non fat milk, 2hrs room temperature

Primary Anitbody

Dilution Ratio1:500 in TBS-T 3% non fat milk, overnight at 4°C

Secondary Antibody

Secondary DescriptionGoat anti rabbit HRP, in TBS-T 3% non fat milk, 1 h at room temperature
Secondary Manufacturer Cat#ab6721
Secondary Concentration1:3000

Details

Detection NotesECL reagent (Pierce), 1 minute incubation, 1 minute exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dectin-1/CLEC7A Antibody (NBP1-45514). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a human dectin-1 and 2 for western blot. How can I know if it works? Do you have any references from the literature that confirm your products? If yes-I'll be happy to read few articles who used your dectin-1 and it worked.
    • Unfortunately, to the best of our knowledge, our dectin antibodies have not been cited in literature yet, and for the peace of your mind, my suggestion to you would be to make your decision based upon the validation images shown on datasheets. You may find our Dectin 1 and Dectin 2 antibodies suitable for WB application using these links. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products. Please let me know if I can help you with any other queries.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Dectin-1/CLEC7A Antibody (NBP1-45514)

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Diseases for Dectin-1/CLEC7A Antibody (NBP1-45514)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

silvia agostini
07/07/2014
Application: IF
Species:

silvia agostini
06/30/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CLEC7A
Entrez
Uniprot