Dectin-1/CLEC7A Antibody (259931) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin-1/CLEC7A PE-conjugated Monoclonal Antibody (Catalog # FAB1859P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 259931
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Dectin-1/CLEC7A Antibody (259931) [Phycoerythrin] Summary

• Additional Information: Clone 259931 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
  Thr66-Met201
  Accession # NP_072092
• Specificity: Detects human Dectin-1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC7A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-1/CLEC7A Antibody (259931) [Phycoerythrin]

• Beta-glucan receptor
• BGR
• CD369
• CLEC7A
• CLECSF12
• CLECSF12DC-associated C-type lectin 1
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
• C-type lectin domain family 7 member A
• C-type lectin domain family 7, member A
• C-type lectin superfamily member 12
• Dectin1
• Dectin-1
• DECTIN1CANDF4
• Dendritic cell-associated C-type lectin 1
• dendritic cell-associated C-type lectin-1
• hDectin-1
• lectin-like receptor 1

Background

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4 + T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
4. Yokota, K. et al. (2001) Gene 272:51.
5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dectin-1/CLEC7A Antibody (FAB1859P)(14)

Publications using FAB1859P

• X Xie, J He, Q Wang, Y Liu, W Chen, K Shi FPR2 participates in epithelial ovarian cancer (EOC) progression through RhoA-mediated M2 macrophage polarization Journal of ovarian research, 2021-12-20;14(1):177. 2021-12-20 [PMID: 34930387] (Flow Cytometry, Human)
• Philipp Kotthoff, Annkristin Heine, Stefanie Andrea Erika Held, Peter Brossart Dexamethasone induced inhibition of Dectin-1 activation of antigen presenting cells is mediated via STAT-3 and NF-?B signaling pathways. Scientific Reports, 2017-07-03;0(0):2045-2322. 2017-07-03 [PMID: 28674449] (Flow Cytometry, Human)
• M Fischer, JP Müller, B Spies-Weis, C Gräfe, O Kurzai, K Hünniger, A Hochhaus, S Scholl, U Schnetzke Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity Eur. J. Immunol, 2017-04-13;0(0):. 2017-04-13 [PMID: 28303575] (Flow Cytometry, Human)
• Mike Fischer, Baerbel Spies-Weisshart, Karin Schrenk, Bernd Gruhn, Susan Wittig, Anita Glaser et al. Polymorphisms of Dectin-1 and TLR2 Predispose to Invasive Fungal Disease in Patients with Acute Myeloid Leukemia PLOS ONE 3/10/2016 [PMID: 26963509]
• Mike Fischer, Baerbel Spies-Weisshart, Karin Schrenk, Bernd Gruhn, Susan Wittig, Anita Glaser et al. Polymorphisms of Dectin-1 and TLR2 Predispose to Invasive Fungal Disease in Patients with Acute Myeloid Leukemia PLOS ONE 2016-03-10 [PMID: 26963509] (Flow Cytometry, Human)
• Clark GJ et al. New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology 1/1/2016 [PMID: 26900474]
• Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015-03-18;10(3):e0119244. 2015-03-18 [PMID: 25786219] (Neutralization, Human)
• Bilin Dong, Dongsheng Li, Ruoyu Li, Sharon C.-A. Chen, Weihuang Liu, Wei Liu et al. A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta -Glucans PLoS ONE 12/9/2014 [PMID: 25490199]
• Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system. PLoS ONE, 2013-12-10;8(12):e82734. 2013-12-10 [PMID: 24340055] (Flow Cytometry, Human)
• Cardone M, Ikeda K, Varano B, Belardelli F, Millefiorini E, Gessani S, Conti L Opposite regulatory effects of IFN-beta and IL-3 on C-type lectin receptors, antigen uptake, and phagocytosis in human macrophages. J Leukoc Biol, 2013-09-09;95(1):161-8. 2013-09-09 [PMID: 24018352] (Flow Cytometry, Human)
• Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production. PLoS ONE, 2012-12-11;7(12):e50589. 2012-12-11 [PMID: 23239982] (Flow Cytometry, Human)
• Khoo AL, Chai LY, Koenen HJ, Oosting M, Steinmeyer A, Zuegel U, Joosten I, Netea MG, van der Ven AJ Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective cathelicidin production. Cytokine, 2011-05-17;55(2):294-300. 2011-05-17 [PMID: 21592820] (Flow Cytometry, Human)
• Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 2010-10-15;134(2):113-21. 2010-10-15 [PMID: 20951740] (Flow Cytometry, Human)
• Cunha C, Di Ianni M, Bozza S, Giovannini G, Zagarella S, Zelante T, D'Angelo C, Pierini A, Pitzurra L, Falzetti F, Carotti A, Perruccio K, Latge JP, Rodrigues F, Velardi A, Aversa F, Romani L, Carvalho A Dectin-1 Y238X polymorphism associates with susceptibility to invasive aspergillosis in hematopoietic transplantation through impairment of both recipient- and donor-dependent mechanisms of antifungal immunity. Blood, 2010-08-31;116(24):5394-402. 2010-08-31 [PMID: 20807886] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CLEC7A
• Uniprot:
  • NP_072092()