Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Nectin-1/PVRL1 Antibody
CLPED1ectodermal dysplasia 4 (Margarita Island type)
Herpes virus entry mediator C
Herpesvirus entry mediator C
Herpesvirus Ig-like receptor
HVECpoliovirus receptor-like 1
poliovirus receptor-related 1 (herpesvirus entry mediator C)
poliovirus receptor-related protein 1
Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+-independent cell‑cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31‑334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4‑7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8‑11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).
Lopez, M. et al. (1995) Gene 155:261.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
Lopez, M. et al. (2001) J. Virol. 75:5684.
Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
Linehan, M. M. et al. (2004) J. Virol. 78:2530.
Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
Togashi, H. et al. (2006) J. Cell Biol. 174:141.
Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Suzuki, K. et al. (2000) Nat. Genet. 25:427.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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