DEC1 Antibody

Images

 
Western Blot: DEC1 Antibody [NB100-1800] - Western Blot Image of anti-DEC1. Whole cell protein from A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: DEC1 Antibody [NB100-1800] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: DEC1 Antibody [NB100-1800] - P. gingivalis-challenged rat maxilla using DEC1 antibody at 1:100. Heat mediated antigen retrieval (sodium citrate buffer pH 6.0) was performed. ...read more
Flow Cytometry: DEC1 Antibody [NB100-1800] - An intracellular stain was performed on A431 cells with DEC1 Antibody NB100-1800AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: DEC1 Antibody [NB100-1800] - Western blot analysis of DEC1 in HeLa whole cell lysate.
Independent Antibodies: Immunoprecipitation: DEC1 Antibody [NB100-1800] - Samples: Whole cell lysate from HeLa (50 ug for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit ...read more
Simple Western: DEC1 Antibody [NB100-1800] - Simple Western lane view shows a specific band for DEC1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Genetic Strategies: Immunoblotting: DEC1 Antibody [NB100-1800] - (B) HSC-2 cells were used to perform knockdown assays for three primary antibodies: HIF1A, DEC1, and DEC2 9. Real-time RT-PCR evaluated expression ...read more
Immunoblotting: DEC1 Antibody [NB100-1800] - DNA-DRR genes promoter activities decreased with DEC. (B) The DEC1 or DEC2 expression plasmids (B), each reporter, and DEC1 or delta basic DEC1 expression plasmids (C) were ...read more
Genetic Strategies: Immunoblotting: DEC1 Antibody [NB100-1800] - Knockdown assays in HSC-2 cells for HIF1A, DEC1, and DEC2. After transfecting and culturing non-specific siRNA or targeted siRNA into HSC-2, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, ChIP, CHIP-SEQ
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

DEC1 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal region of human DEC1 (between amino acids 350-412) [UniProt O14503]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BHLHE40
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Simple Western 1:50
  • ELISA
  • Flow Cytometry 2-5 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation Sequencing
Application Notes
This DEC1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen approx. 49 kDa. In ICC/IF punctate nuclear staining is observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29715265). Use in Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID: 31061528). Use in ELISA reported in scientific literature (PMID: 31061528).
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DEC1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1800 in the following applications:

Publications
Read Publications using
NB100-1800 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26834156).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DEC1 Antibody

  • basic helix-loop-helix domain containing, class B, 2
  • basic helix-loop-helix family, member e40
  • bHLHb2
  • bHLHe40
  • Class B basic helix-loop-helix protein 2
  • class E basic helix-loop-helix protein 40
  • DEC1HLHB2
  • differentially expressed in chondrocytes 1
  • Differentially expressed in chondrocytes protein 1
  • differentiated embryo chondrocyte expressed gene 1
  • Enhancer-of-split and hairy-related protein 2
  • SHARP2
  • SHARP-2
  • Stimulated by retinoic acid gene 13 protein
  • STRA13FLJ99214
  • Stra14

Background

The basic helix-loop-helix (bHLH) transcription factor DEC1 (differentially expressed in chondrocytes protein 1) is wildly expressed in most normal tissues in a proportion of cells, whereas in tumors, its expression gets upregulated (potentially by hypoxia) in several malignancies such as breast, stomach, colon, lung, liver cancer and pancreatic ductal adenocarcinoma. The gene expression of DEC1 is regulated by various extracellular stimuli in a cell type-specific manner. Localized in the nucleus, it can homodimerize or heterodimerize with TCF3/E47, and interacts with ubiquitin-conjugating enzyme UBE2I/UBC9. DEC1 has emerged as one of the major circadian molecules alongwith its involvement in homeostasis of metabolism and energy. DEC1 has been shown to play a key role in various biological phenomena such as circadian rhythms, neurogenesis, neuroregulation, chondrogenesis, cellular growth, carcinogenesis, immune response balance as well as cellular senescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1800
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, ChIP, CHIP-SEQ

Publications for DEC1 Antibody (NB100-1800)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: CHIP-SEQ, ChIP, IHC, WB.


Filter By Application
CHIP-SEQ
(1)
ChIP
(3)
IHC
(3)
WB
(15)
All Applications
Filter By Species
Human
(10)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-1800 Applications Species
Sato F, Bhawal U, Sugiyama N et al. Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis. Clocks&Sleep Jan 3 2020 [PMID: 33089188] (IHC, WB, Human) IHC, WB Human
Pasula S, Tessneer KL, Fu Y, et al. Risk Variants with Opposing Functional Effects Result in Hypomorphic Expression of TNIP1 and Other Genes within a 3D Chromatin Network Arthritis & rheumatology (Hoboken, N.J.) Dec 5 2019 [PMID: 31804013] (ChIP, WB, Human) ChIP, WB Human
Le HT, Sato F, Kohsaka A et al. Dec1 Deficiency Suppresses Cardiac Perivascular Fibrosis Induced by Transverse Aortic Constriction Int J Mol Sci 2019 Oct 08 [PMID: 31597354] (IHC, Mouse) IHC Mouse
Li C, Zhu B, Son YM et al. The Transcription Factor Bhlhe40 Programs Mitochondrial Regulation of Resident CD8+ T Cell Fitness and Functionality Immunity [PMID: 31533057]
Rauschmeier R, Gustafsson C Bhlhe40 and Bhlhe41 transcription factors regulate alveolar macrophage self-renewal and identity. EMBO J Jan 10 2019 [PMID: 31414712] (ChIP, Mouse) ChIP Mouse
Jarjour NN, Schwarzkopf EA, Bradstreet TR, Shchukina I et al. Bhlhe40 mediates tissue-specific control of macrophage proliferation in homeostasis and type 2 immunity Nat. Immunol. Jun 1 2019 [PMID: 31061528] (CHIP-SEQ, Mouse) CHIP-SEQ Mouse
Sato F, Otsuka T, Kohsaka A et al. Smad3 suppresses epithelial cell migration and proliferation via the clock gene Dec1, which negatively regulates the expression of clock genes Dec2 and Per1. Am. J. Pathol. Jan 18 2019 [PMID: 30664860] (WB, Mouse) WB Mouse
Huynh JP, Lin CC, Kimmey JM et al. Bhlhe40 is an essential repressor of IL-10 during Mycobacterium tuberculosis infection J. Exp. Med. May 17 2018 [PMID: 29773644] (WB, Mouse) WB Mouse
Hamilton KA, Wang Y, Raefsky SM et al. Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus PLoS ONE May 1 2018 [PMID: 29715265] (ChIP, Mouse) ChIP Mouse
Nakamura H, Bono H, Hiyama K, Kawamoto T. Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE. 2018 Feb 21 [PMID: 29466367] (WB, Human) WB Human
Show All 24 Publications.

Reviews for DEC1 Antibody (NB100-1800) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NB100-1800:
Filter by Applications
IHC-P
(1)
ChIP
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin DEC1 NB100-1800
Enlarge
5
reviewed by:
UJJAL BHAWAL
IHC-P Rat 06/28/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPorphyromonas gingivalis-challenged rat maxilla
SpeciesRat
LotC-2
Chromatin Immunoprecipitation DEC1 NB100-1800
Enlarge
5
reviewed by:
Anonymous
ChIP Human 07/07/2016
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedRenal cell line
SpeciesHuman

Details

Detection NotesSequencing
DNS SharingBioruptor

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DEC1 Antibody (NB100-1800) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1800AF350
Alexa Fluor 405 NB100-1800AF405
Alexa Fluor 488 NB100-1800AF488
Alexa Fluor 532 NB100-1800AF532
Alexa Fluor 594 NB100-1800AF594
Alexa Fluor 647 NB100-1800AF647
Alexa Fluor 700 NB100-1800AF700
Alexa Fluor 750 NB100-1800AF750
Biotin NB100-1800B
DyLight 350 NB100-1800UV
DyLight 405 NB100-1800V
DyLight 488 NB100-1800G
DyLight 550 NB100-1800R
DyLight 594 NB100-1800DL594
DyLight 650 NB100-1800C
DyLight 680 NB100-1800FR
DyLight 755 NB100-1800IR
FITC NB100-1800F
HRP NB100-1800H
Janelia Fluor 549 NB100-1800JF549
Janelia Fluor 646 NB100-1800JF646

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Bioinformatics Tool for DEC1 Antibody (NB100-1800)

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

UJJAL BHAWAL
06/28/2018
Application: IHC-P
Species: Rat

Anonymous
07/07/2016
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol BHLHE40
Entrez
Uniprot