DEC1 Antibody - BSA Free

Images

 
Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - Western Blot Image of anti-DEC1. Whole cell protein from A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: DEC1 Antibody - BSA Free [NB100-1800] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry: DEC1 Antibody - BSA Free [NB100-1800] - Immunohistochemical detection of DEC1 in myocardial and stromal cells. Representative images of one WT heart treated with TAC and sham at four weeks. The ...read more
Flow Cytometry: DEC1 Antibody - BSA Free [NB100-1800] - An intracellular stain was performed on A431 cells with DEC1 Antibody NB100-1800AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Biological Strategies: Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - DEC1 expression decreases in HeLa cells under apoptosis. Western blotting images of cleaved-poly (ADP-ribose) polymerase (PARP), ...read more
Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - Western blot analysis of DEC1 in HeLa whole cell lysate.
Immunohistochemistry-Paraffin: DEC1 Antibody - BSA Free [NB100-1800] - P. gingivalis-challenged rat maxilla using DEC1 antibody at 1:100. Heat mediated antigen retrieval (sodium citrate buffer pH 6.0) was performed. ...read more
Immunoprecipitation: DEC1 Antibody - BSA Free [NB100-1800] - Samples: Whole cell lysate from HeLa (50 ug for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody ...read more
Simple Western: DEC1 Antibody - BSA Free [NB100-1800] - Simple Western lane view shows a specific band for DEC1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunoblotting: DEC1 Antibody - BSA Free [NB100-1800] - (B) HSC-2 cells were used to perform knockdown assays for three primary antibodies: HIF1A, DEC1, and DEC2 9. Real-time RT-PCR evaluated expression levels of MSH2, ...read more
Immunoblotting: DEC1 Antibody - BSA Free [NB100-1800] - DNA-DRR genes promoter activities decreased with DEC. (B) The DEC1 or DEC2 expression plasmids (B), each reporter, and DEC1 or delta basic DEC1 expression plasmids ...read more
Immunoblotting: DEC1 Antibody - BSA Free [NB100-1800] - Knockdown assays in HSC-2 cells for HIF1A, DEC1, and DEC2. After transfecting and culturing non-specific siRNA or targeted siRNA into HSC-2, culturing them under ...read more
p27S10 phosphorylation is required for PHD3 depletion induced increase in p27 half-life. a Quantification of p27S10 phosphorylation from three independent experiments in 786-O cells. S10 phosphorylation demonstrated ...read more
OTULIN overexpression reduced the number of Iba-1+ microglial cells in the cortical ischemic penumbra in focal cerebral ischemia/reperfusion rats. A Representative images of Iba-1+ microglial cells that were ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, WB, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

DEC1 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to a C-terminal region of human DEC1 (between amino acids 350-412) [UniProt O14503]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BHLHE40
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) reported in scientific literature (PMID 31061528)
  • Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 31061528)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 29715265)
  • ELISA reported in scientific literature (PMID 31061528)
  • Flow Cytometry 2-5 ug/ml
  • Immunoblotting 1:500-1:1500
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation
  • Simple Western 1:50
  • Western Blot 1:500-1:1500
Application Notes
In Western Blot, a band is seen approx. 49 kDa. In ICC/IF punctate nuclear staining is observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DEC1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1800 in the following applications:

Publications
Read Publications using
NB100-1800 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26834156).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DEC1 Antibody - BSA Free

  • basic helix-loop-helix domain containing, class B, 2
  • basic helix-loop-helix family, member e40
  • bHLHb2
  • bHLHe40
  • Class B basic helix-loop-helix protein 2
  • class E basic helix-loop-helix protein 40
  • DEC1HLHB2
  • differentially expressed in chondrocytes 1
  • Differentially expressed in chondrocytes protein 1
  • differentiated embryo chondrocyte expressed gene 1
  • Enhancer-of-split and hairy-related protein 2
  • SHARP2
  • SHARP-2
  • Stimulated by retinoic acid gene 13 protein
  • STRA13FLJ99214
  • Stra14

Background

The basic helix-loop-helix (bHLH) transcription factor DEC1 (differentially expressed in chondrocytes protein 1) is wildly expressed in most normal tissues in a proportion of cells, whereas in tumors, its expression gets upregulated (potentially by hypoxia) in several malignancies such as breast, stomach, colon, lung, liver cancer and pancreatic ductal adenocarcinoma. The gene expression of DEC1 is regulated by various extracellular stimuli in a cell type-specific manner. Localized in the nucleus, it can homodimerize or heterodimerize with TCF3/E47, and interacts with ubiquitin-conjugating enzyme UBE2I/UBC9. DEC1 has emerged as one of the major circadian molecules alongwith its involvement in homeostasis of metabolism and energy. DEC1 has been shown to play a key role in various biological phenomena such as circadian rhythms, neurogenesis, neuroregulation, chondrogenesis, cellular growth, carcinogenesis, immune response balance as well as cellular senescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1800
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, WB, ChIP

Publications for DEC1 Antibody (NB100-1800)(41)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: ChIP, Chemotaxis, Chip Cytometry, FLOW, IF/IHC, IHC, IHC-P, Simple Western, WB.


Filter By Application
ChIP
(2)
Chemotaxis
(3)
Chip Cytometry
(1)
FLOW
(1)
IF/IHC
(4)
IHC
(1)
IHC-P
(1)
Simple Western
(1)
WB
(18)
All Applications
Filter By Species
Human
(14)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NB100-1800 Applications Species
Montalvo AP, Gruskin ZL, Leduc A et al. An adult clock component links circadian rhythms to pancreatic ?-cell maturation bioRxiv 2023-08-17 [PMID: 37609178] (FLOW) FLOW
Chen Z, Javed N, Moore M et al. Integrative dissection of gene regulatory elements at base resolution Cell genomics 2023-06-14 [PMID: 37388913] (ChIP, Human) ChIP Human
Lv J, Wei Y, Yin JH et al. The tumor immune microenvironment of nasopharyngeal carcinoma after gemcitabine plus cisplatin treatment Nature medicine 2023-06-01 [PMID: 37280275]
Pathmanapan, S;Poon, R;De Renshaw, TB;Nadesan, P;Nakagawa, M;Seesankar, GA;Ho Loe, AK;Zhang, HH;Guinovart, JJ;Duran, J;Newgard, CB;Wunder, JS;Alman, BA; Mutant IDH regulates glycogen metabolism from early cartilage development to malignant chondrosarcoma formation Cell reports 2023-06-01 [PMID: 37267108] (IHC, Human, Mouse) IHC Human, Mouse
Nechanitzky R, Nechanitzky D, Ramachandran P et al. Cholinergic control of Th17 cell pathogenicity in experimental autoimmune encephalomyelitis Cell death and differentiation 2022-12-17 [PMID: 36528755] (ChIP, Mouse) ChIP Mouse
Hu X, Zou M, Ni L et al. Dec1 Deficiency Ameliorates Pulmonary Fibrosis Through the PI3K/AKT/GSK-3beta/beta-Catenin Integrated Signaling Pathway Frontiers in pharmacology 2022-03-09 [PMID: 35355710] (WB, Mouse, Human) WB Mouse, Human
Al-chalabi SA Long Noncoding RNA AK001796 as a Mediator of Epithelial-to-Mesenchymal Transition in Metastatic Breast Cancer Thesis 1905-07-13
Shearn, CT;Anderson, AL;Devereaux, MW;El Kasmi, KC;Orlicky, DJ;Sokol, RJ; Expression of circadian regulatory genes is dysregulated by increased cytokine production in mice subjected to concomitant intestinal injury and parenteral nutrition PloS one 2023-08-30 [PMID: 37647292] (WB) WB
Karakaya S, Gunnesson L, Elias E et al. Cytoplasmic HIF-2? as tissue biomarker to identify metastatic sympathetic paraganglioma Scientific reports 2023-07-18 [PMID: 37463949] (IHC-P, Human) IHC-P Human
Centeno PP, Pavet V, Marais R The journey from melanocytes to melanoma Nature reviews. Cancer 2023-06-01 [PMID: 37095242]
Show All 41 Publications.

Reviews for DEC1 Antibody (NB100-1800) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NB100-1800:
Filter by Applications
IHC-P
(1)
ChIP
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin DEC1 NB100-1800
Enlarge
5
reviewed by:
UJJAL BHAWAL
IHC-P Rat 06/28/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPorphyromonas gingivalis-challenged rat maxilla
SpeciesRat
LotC-2
Chromatin Immunoprecipitation DEC1 NB100-1800
Enlarge
5
reviewed by:
Verified Customer
ChIP Human 07/07/2016
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedRenal cell line
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for DEC1 Antibody (NB100-1800) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Pathways for DEC1 Antibody (NB100-1800)

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Research Areas for DEC1 Antibody (NB100-1800)

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

UJJAL BHAWAL
06/28/2018
Application: IHC-P
Species: Rat

Verified Customer
07/07/2016
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol BHLHE40
Entrez
Uniprot