CRY1 Antibody [Unconjugated]

CRY1 was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Goat Anti-Human/Mouse CRY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3764) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CRY1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CRY1
  Lys115-His224
  Accession # Q16526
• Specificity: Detects human and mouse CRY1 in direct ELISAs and Western Blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRY1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRY1 Antibody [Unconjugated]

• CRY1
• cryptochrome 1 (photolyase-like)
• Cryptochrome I
• PHLL1
• PHLL1cryptochrome-1
• photolyase-like cryptochrome 1

Background

CRY1 is a critical component of the circadian oscillator that represses CLOCK:BMAL1 mediated transcription when translocated into the nucleus. It is a member of the DNA photolyase family but has no photolyase activity. Over the region used as immunogen, human and mouse CRY1 share 94% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRY1 Antibody (AF3764)(1)

Publication using AF3764

• B Kim, J Kim, M Chun, I Park, D Kwak, M Choi, K Kim, HK Choe Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes Scientific Reports, 2021-01-28;11(1):2575. 2021-01-28 [PMID: 33510438] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CRY1
• Uniprot:
  • Q16526()