DEC2/SHARP1 Antibody

Images

 
Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Huh7 cell lysates loaded from left to right at concentrations of 20, 30, and 40 ug of total protein. Image from verified customer review.
Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody [NBP1-19613] - Staining of SHARP1 in mouse pancreas.
Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of SHARP1 in HeLa whole cell extract.
Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of HeLa (1), Raw 264.7 (2), A431 (3), MCF7 (4), and PC12 (5) using SHARP1 antibody at 2 ug/mL.
Simple Western: DEC2/SHARP1 Antibody [NBP1-19613] - Image shows a specific band for SHARP1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

DEC2/SHARP1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human DEC2/SHARP1 inbetween residues 1-75 [UniProt Q9C0J9].
Localization
Nucleus
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BHLHE41
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DEC2/SHARP1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-19613 in the following applications:

Publications
Read Publications using
NBP1-19613 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human, Mouse, Rat. Immunogen has 100% homology to bovine and canine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DEC2/SHARP1 Antibody

  • basic helix-loop-helix domain containing, class B, 3
  • basic helix-loop-helix family, member e41
  • BHLHB3
  • BHLHB3class E basic helix-loop-helix protein 41
  • BHLHE41
  • Class B basic helix-loop-helix protein 3
  • DEC2
  • DEC2Enhancer-of-split and hairy-related protein 1
  • Differentially expressed in chondrocytes protein 2
  • SHARP-1
  • SHARP1hDEC2

Background

SHARP1 also known as DEC2 and BHLHe41 encodes a transcription factor that belongs to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors (bHLH). However, sequence alignment shows that SHARP-1 is only distantly related to these proteins, exhibiting 37-42% sequence identity within the bHLH domain. Unlike most bHLH proteins, SHARP-1 is not expressed in neuronal progenitor cells or early differentiating neurons but is restricted to a subset of neurons of the postnatal central nervous system. Recent studies have shown DEC2 plays a regulatory role in clockwork system along with Clock and Bmal1. DEC1, a structurally related protein also known as SHARP2 along with DEC2 works as the negative regulated of molecular clock. Mutations in this gene (hDEC2-P385R) are associated with short sleep phenotypes in human and transgenic mice carrying this mutation show increased vigilance time and less sleep time than control mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DEC2/SHARP1 Antibody (NBP1-19613)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(2)
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Filter By Species
Human
(2)
All Species

Review for DEC2/SHARP1 Antibody (NBP1-19613) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-19613:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot DEC2/SHARP1 NBP1-19613
Enlarge
4
reviewed by:
Anonymous
WB Human 01/25/2019
View

Summary

ApplicationWestern Blot
Sample TestedHuh7 cell lysate
SpeciesHuman
LotA-2

Product General Protocols

View specific protocols for DEC2/SHARP1 Antibody (NBP1-19613): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DEC2/SHARP1 Antibody (NBP1-19613). (Showing 1 - 1 of 1 FAQs).

  1. Your data sheet shows the sharp-1 MW at around 50KDa. However, when I do Western blot using extract from breast cancer cell lines, I see a band around 35, why the molecular weight of this protein is different on your datasheet?
    • The expected weight of Sharp-1 is 50 kDa according to UniProt Q9C0J9. However, if the 35 kDa band you are seeing is consistent with what other companies see, then I would believe that it is the correct band. The 35 kDa band you and other companies are seeing is most likely a degradation product. You may also want to consult the published literature to confirm.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-19613AF350
Alexa Fluor 405 NBP1-19613AF405
Alexa Fluor 488 NBP1-19613AF488
Alexa Fluor 532 NBP1-19613AF532
Alexa Fluor 594 NBP1-19613AF594
Alexa Fluor 647 NBP1-19613AF647
Alexa Fluor 700 NBP1-19613AF700
Alexa Fluor 750 NBP1-19613AF750
Biotin NBP1-19613B
DyLight 350 NBP1-19613UV
DyLight 405 NBP1-19613V
DyLight 488 NBP1-19613G
DyLight 550 NBP1-19613R
DyLight 594 NBP1-19613DL594
DyLight 650 NBP1-19613C
DyLight 680 NBP1-19613FR
DyLight 755 NBP1-19613IR
FITC NBP1-19613F
HRP NBP1-19613H
Janelia Fluor 549 NBP1-19613JF549
Janelia Fluor 646 NBP1-19613JF646

Additional DEC2/SHARP1 Products

Bioinformatics Tool for DEC2/SHARP1 Antibody (NBP1-19613)

Discover related pathways, diseases and genes to DEC2/SHARP1 Antibody (NBP1-19613). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for DEC2/SHARP1 Antibody (NBP1-19613)

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Blogs on DEC2/SHARP1.

SHARP1 - An enhancer-of-split- and hairy-related protein
SHARP1 encodes a transcription repressor factor that belongs to the Hairy/Enhancer of the Split subfamily of basic helix-loop-helix factors (bHLH). Sequence alignment shows that SHARP1 is only distantly related to these proteins with a 37-42% sequence...  Read full blog post.

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
01/25/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol BHLHE41
Entrez
Uniprot