DEC2/SHARP1 Antibody

Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Huh7 cell lysates loaded from left to right at concentrations of 20, 30, and 40 ug of total protein. Image from verified customer review.

Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody [NBP1-19613] - Staining of SHARP1 in mouse pancreas.

Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of SHARP1 in HeLa whole cell extract.

Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of HeLa (1), Raw 264.7 (2), A431 (3), MCF7 (4), and PC12 (5) using SHARP1 antibody at 2 ug/mL.

Simple Western: DEC2/SHARP1 Antibody [NBP1-19613] - Image shows a specific band for SHARP1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, Simple Western, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DEC2/SHARP1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human DEC2/SHARP1 inbetween residues 1-75 [UniProt Q9C0J9].
• Localization: Nucleus
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BHLHE41
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  DEC2/SHARP1 Knockout HeLa Cell Lysate

Reactivity Notes

Human, Mouse, Rat. Immunogen has 100% homology to bovine and canine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DEC2/SHARP1 Antibody

• basic helix-loop-helix domain containing, class B, 3
• basic helix-loop-helix family, member e41
• BHLHB3
• BHLHB3class E basic helix-loop-helix protein 41
• BHLHE41
• Class B basic helix-loop-helix protein 3
• DEC2
• DEC2Enhancer-of-split and hairy-related protein 1
• Differentially expressed in chondrocytes protein 2
• SHARP-1
• SHARP1hDEC2

Background

SHARP1 also known as DEC2 and BHLHe41 encodes a transcription factor that belongs to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors (bHLH). However, sequence alignment shows that SHARP-1 is only distantly related to these proteins, exhibiting 37-42% sequence identity within the bHLH domain. Unlike most bHLH proteins, SHARP-1 is not expressed in neuronal progenitor cells or early differentiating neurons but is restricted to a subset of neurons of the postnatal central nervous system. Recent studies have shown DEC2 plays a regulatory role in clockwork system along with Clock and Bmal1. DEC1, a structurally related protein also known as SHARP2 along with DEC2 works as the negative regulated of molecular clock. Mutations in this gene (hDEC2-P385R) are associated with short sleep phenotypes in human and transgenic mice carrying this mutation show increased vigilance time and less sleep time than control mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEC2/SHARP1 Antibody (NBP1-19613)(2)

Publications using NBP1-19613

• Liao Y, Lu W, Che Q et al. SHARP1 Suppresses Angiogenesis of Endometrial Cancer by Decreasing Hypoxia-Inducible Factor-1a Level PLoS ONE. 2014 Jun 12 [PMID: 24918449] (WB, IHC-P, Human)
  
  Details:
  SHARP1 antibody used for IHC-P (1:100 to 10 µg/ml dilution, incubated at 4C ON) on endometrial cancer or normal endometrium from human subjects and nude mice tumor tissues (staining images in Figure 1A-F, Figure 5F). WB was performed (1:500 to 2 µg/ml dilution, incubated 4C ON) on Human EC cell lines (Ishikawa and RL95-2) - Ishikawa and RL95-2 cells (Figure 2A), vector/SHARP1-overexpressing Ishikawa cells (Figure 2B, Figure 5A, Figure-S1B) and SHARP1 depleted RL95-2 cells under normoxic/hypoxic conditions (Figure 2C, Figure-S1D), Co-immunoprecipitation of endogenous HIF-1 alpha -SHARP1 in Ishikawa cells (Figure 2E) and Ishikawa cells exposed to normoxia/hypoxia (Figure 2F). Figure- S2 shows the WB of SHARP1 in siRNA-HIF1 alpha depleted Ishikawa cells.
• Inaguma S, Riku M, Hashimoto M et al. GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through BHLHE41-mediated suppression of MLH1. Cancer Res. 2013 Oct 28 [PMID: 24165159] (WB, Human)

Review for DEC2/SHARP1 Antibody (NBP1-19613) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 01/25/2019

Summary

• Application: Western Blot
• Sample Tested: Huh7 cell lysate
• Species: Human
• Lot: A-2

FAQs for DEC2/SHARP1 Antibody (NBP1-19613). (Showing 1 - 1 of 1 FAQs).

1. Your data sheet shows the sharp-1 MW at around 50KDa. However, when I do Western blot using extract from breast cancer cell lines, I see a band around 35, why the molecular weight of this protein is different on your datasheet?
   • The expected weight of Sharp-1 is 50 kDa according to UniProt Q9C0J9. However, if the 35 kDa band you are seeing is consistent with what other companies see, then I would believe that it is the correct band. The 35 kDa band you and other companies are seeing is most likely a degradation product. You may also want to consult the published literature to confirm.

Bioinformatics

• Gene Symbol: BHLHE41
• Entrez:
  • 79365(Human)
  • 79362(Mouse)
  • 117095(Rat)
• Uniprot:
  • Q9C0J9()