HSD17B4 Antibody (OTI4C4)

Images

 
Western Blot: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Ovary tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Kidney tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human liver tissue.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HeLa cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA, ICC/IF
Clone
OTI4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSD17B4 Antibody (OTI4C4) Summary

Immunogen
Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSD17B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:4000
Application Notes
Use in Proximity Ligation Assay and Immunoprecipitation reported in scientific literature (PMID: 31176039).
Reviewed Applications
Read 1 Review rated 4
using
NBP2-46005 in the following applications:

Publications
Read Publications using
NBP2-46005 in the following applications:

  • IP
    1 publication
  • PLA
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31176039). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSD17B4 Antibody (OTI4C4)

  • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
  • 7-alpha
  • D-bifunctional protein
  • EDH17B4
  • hydroxysteroid (17-beta) dehydrogenase 4
  • MPF-2
  • peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
  • peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
  • peroxisomal
  • short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Background

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSD17B4 Antibody (NBP2-46005)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IP, PLA.


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IP
(1)
PLA
(1)
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Review for HSD17B4 Antibody (NBP2-46005) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-46005:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot HSD17B4 NBP2-46005
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 02/01/2017
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Summary

ApplicationWestern Blot
Sample TestedN2a and HEK293 T cell lysates
SpeciesMouse

Comments

CommentsWe first test this antibody in HEK293T (Human Embryonic Kidney Cells) and N2a (mouse neuroblastoma cell line) cell lysis. Both of them show the positive bands when NBP2-46005 is used.
Then we do the IP experiments, the bait is the interacting protein of HSD17B4 coated beads which are incubated with N2a cell lysates, we also set the control which is free of any interacting protein of HSD17B4. It can be detected in the blot that input and bait both show the positive bands of HSD17B4 but not control.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSD17B4 Antibody (NBP2-46005) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
02/01/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HSD17B4