HSD17B4 Antibody (OTI4C4)

Images

 
Western Blot: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Ovary tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Kidney tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human liver tissue.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HeLa cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP, PLA, ICC/IF
Clone
OTI4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSD17B4 Antibody (OTI4C4) Summary

Immunogen
Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSD17B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:4000
Application Notes
Use in Proximity Ligation Assay and Immunoprecipitation reported in scientific literature (PMID: 31176039).
Reviewed Applications
Read 1 Review rated 4
using
NBP2-46005 in the following applications:

Publications
Read Publications using
NBP2-46005 in the following applications:

  • IP
    1 publication
  • PLA
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31176039). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSD17B4 Antibody (OTI4C4)

  • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
  • 7-alpha
  • D-bifunctional protein
  • EDH17B4
  • hydroxysteroid (17-beta) dehydrogenase 4
  • MPF-2
  • peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
  • peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
  • peroxisomal
  • short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSD17B4 Antibody (NBP2-46005)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IP, PLA.


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IP
(1)
PLA
(1)
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Review for HSD17B4 Antibody (NBP2-46005) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-46005:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot HSD17B4 NBP2-46005
Enlarge
4
reviewed by:
ZHU ZHIHUI
WB Mouse 02/01/2017
View

Summary

ApplicationWestern Blot
Sample TestedN2a and HEK293 T cell lysates
SpeciesMouse

Comments

CommentsWe first test this antibody in HEK293T (Human Embryonic Kidney Cells) and N2a (mouse neuroblastoma cell line) cell lysis. Both of them show the positive bands when NBP2-46005 is used.
Then we do the IP experiments, the bait is the interacting protein of HSD17B4 coated beads which are incubated with N2a cell lysates, we also set the control which is free of any interacting protein of HSD17B4. It can be detected in the blot that input and bait both show the positive bands of HSD17B4 but not control.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HSD17B4 Antibody (NBP2-46005) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70947AF350
Alexa Fluor 405 NBP2-70947AF405
Alexa Fluor 488 NBP2-70947AF488
Alexa Fluor 532 NBP2-70947AF532
Alexa Fluor 594 NBP2-70947AF594
Alexa Fluor 647 NBP2-70947AF647
Alexa Fluor 700 NBP2-70947AF700
Alexa Fluor 750 NBP2-70947AF750
Biotin NBP2-70947B
DyLight 350 NBP2-70947UV
DyLight 405 NBP2-70947V
DyLight 488 NBP2-70947G
DyLight 550 NBP2-70947R
DyLight 594 NBP2-70947DL594
DyLight 650 NBP2-70947C
DyLight 680 NBP2-70947FR
DyLight 755 NBP2-70947IR
FITC NBP2-70947F
HRP NBP2-70947H
Janelia Fluor 549 NBP2-70947JF549
Janelia Fluor 646 NBP2-70947JF646

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

ZHU ZHIHUI
02/01/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HSD17B4