DDX54 Antibody - BSA Free

Immunoprecipitation: DDX54 Antibody [NB100-60678] - Detection of Human DDX54 on HeLa whole cell lysate using NB100-60678. DDX54 was also immunoprecipitated by rabbit anti-DDX54 antibody NB100-60677.

• Reactivity: Hu, Mu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DDX54 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX54 Antibody - BSA Free (NB100-60678) is a polyclonal antibody validated for use in WB and IP. Anti-DDX54 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 800 and 850 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 using the numbering given in entry NP_076977.3 (GeneID 79039).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX54
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
• Application Notes: Western Blot Not recommended, use rabbit anti-DDX54 antibody NB100-60677.

Reactivity Notes

Mouse reactivity reported in (PMID: 22028794).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX54 Antibody - BSA Free

• APR-5
• ATP-dependent RNA helicase DP97
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
• DEAD box helicase 97 KDa
• DEAD box protein 54
• DEAD box RNA helicase 97 kDa
• DP97ATP-dependent RNA helicase DDX54
• EC 3.6.1
• EC 3.6.4.13
• MGC2835

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX54 Antibody (NB100-60678)(3)

Publications using NB100-60678

• Cottrell KA, Ryu S, Pierce JR et Al. Induction of Viral Mimicry Upon Loss of DHX9 and ADAR1 in Breast Cancer Cells Cancer Res Commun 2024-04-01 [PMID: 38530197]
• Cottrell KA, Ryu S, Torres LS et al. Induction of viral mimicry upon loss of DHX9 and ADAR1 in breast cancer cells bioRxiv : the preprint server for biology 2023-03-01 [PMID: 36909617] (IP, WB)
• Kajiro M, Tsuchiya M, Kawabe Y et al. The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis. PLoS One. 2011-01-01 [PMID: 22028794] (Mouse)

Bioinformatics

• Gene Symbol: DDX54
• Entrez:
  • 79039(Human)
• Uniprot:
  • NP_076977.3(Human)