Cytokeratin 18 Antibody (C-04) - BSA Free

Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.

Flow Cytometry: Cytokeratin 18 Antibody (C-04) [NB500-306] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-cytokeratin 18 (C-04) FITC.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.

• Reactivity: Ma
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: C-04
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytokeratin 18 Antibody (C-04) - BSA Free Summary

• Immunogen: Cytoskeleton preparation of epidermal carcinoma cell line A431.
• Marker: Epithelial Marker
• Specificity: The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:2000
  • Flow (Intracellular) 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1-2 ug/ml
• Application Notes: Predicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. This antibody is CyTOF ready.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 18 Antibody (C-04) - BSA Free

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 18 Antibody (NB500-306)(4)

Publications using NB500-306

• Yang KS, O'Shea A, Zelga P et al. Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma Scientific reports 2023-07-06 [PMID: 37414831] (ELISA, Human)
• Chen M, Pekosz A, Villano JS et al. Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants bioRxiv : the preprint server for biology 2022-04-12 [PMID: 35441175] (IF/IHC)
• Li Z, Wei M, Shen W et al. Sox2 regulates globose basal cell regeneration in the olfactory epithelium International forum of allergy & rhinology 2021-09-26 [PMID: 34569176]
• Kaenel P, Antonijevic M, Richter S et al. Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway. Int J Oncol. 2012-02-01 [PMID: 22020958] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: KRT18