DDX54 Antibody

Western Blot: DDX54 Antibody [NB100-60677] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DDX54 antibody NB100-60677 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. DDX54 was also immunoprecipitated by rabbit anti-DDX54 antibody NB100-60678, which recognizes a downstream epitope.

Immunohistochemistry-Paraffin: DDX54 Antibody [NB100-60677] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX54 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 625 and 675 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 using the numbering given in entry NP_076977.3 (GeneID 79039).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX54
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX54 Antibody

• APR-5
• ATP-dependent RNA helicase DP97
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
• DEAD box helicase 97 KDa
• DEAD box protein 54
• DEAD box RNA helicase 97 kDa
• DP97ATP-dependent RNA helicase DDX54
• EC 3.6.1
• EC 3.6.4.13
• MGC2835

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX54
• Entrez:
  • 79039(Human)
• Uniprot:
  • NP_076977.3(Human)