DDX17 Antibody (JE35-01)

Western Blot: DDX17 Antibody (JE35-01) [NBP3-32261] - Western blot analysis of DDX17 on different lysates with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-OV-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 80 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: DDX17 Antibody (JE35-01) [NBP3-32261] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32261) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: DDX17 Antibody (JE35-01) [NBP3-32261] - Immunocytochemistry analysis of MCF7 cells labeling DDX17 with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: DDX17 Antibody (JE35-01) [NBP3-32261] - Flow cytometric analysis of MCF7 cells labeling DDX17. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32261, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE35-01
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DDX17 Antibody (JE35-01) Summary

• Description: Novus Biologicals Rabbit DDX17 Antibody (JE35-01) (NBP3-32261) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human DDX17 aa 100-200. (Uniprot: Q92841)
• Localization: Nucleus, nucleolus, Cytoplasm, cytosol.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDX17
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DDX17 Antibody (JE35-01)

• DDX17
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• DEAD box protein 17
• DEAD box protein p72
• DEAD/H box 17
• DKFZp761H2016
• EC 3.6.1
• EC 3.6.4.13
• p72
• P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
• probable ATP-dependent RNA helicase DDX17
• RH70
• RNA-dependent helicase p72

Background

DDX17 (p72) is a highly related member of the DEAD box family and is an established RNA helicase. It has been implicated in growth regulation and has been shown to be involved in both pre-mRNA and pre-rRNA processing. This protein has also been reported to act as a transcriptional co-activator for estrogen-receptor alpha (ER ) and shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX17