DDX1 Antibody

Western Blot: DDX1 Antibody [NB200-348] - Whole cell lysate (20, 40 and 80 ug) from HeLa cells. Antibody used at 0.03 ug/ml.

Immunohistochemistry: DDX1 Antibody [NB200-348] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX1 Antibody Summary

• Description: Novus Biologicals Rabbit DDX1 Antibody (NB200-348) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows. Western Blot: 1:1,000 to 1:10,000. In some cases, the antibody may be diluted further than indicated.
• Control:

  HeLa Whole Cell Lysate
  DDX1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX1 Antibody

• ATP-dependent RNA helicase DDX1
• DBP-RB
• DBP-RBDEAD box polypeptide 1
• DDX1
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• DEAD box protein 1
• DEAD box protein retinoblastoma
• DEAD Box-1
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
• DEAD/H-Box Helicase 1
• EC 3.6.1
• EC 3.6.1.10
• EC 3.6.4.13
• UKVH5d

Background

DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX1
• Entrez:
  • 1653(Human)