RNA Helicase A Antibody

Images

 
Biological Strategies: Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody [NB110-40579] - Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is ...read more
Immunohistochemistry: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB
Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 on HeLa whole cell lystate using NB110-40579.
Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by Western blot of immunoprecipitates. Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

RNA Helicase A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1220 and the C-terminus (residue 1270) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 9 using the numbering given in entry CAA71668.1 (GeneID 1660).
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockdown Validated
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25951186). Use in Knockdown reported in scientific literature (PMID: 30111593). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. This RNA Helicase A Antibody is validated for ICC/IF from a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NB110-40579 in the following applications:

Publications
Read Publications using
NB110-40579 in the following applications:

  • 1 publication
  • IP
    1 publication
  • KD
    1 publication
  • WB
    2 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan. Mouse reactivity reported in scientific literature (PMID: 24270455).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNA Helicase A Antibody

  • DDX9ATP-dependent RNA helicase A
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNAhelicase II; leukophysin)
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9
  • DEAH (Asp-Glu-Ala-His) box polypeptide 9
  • DEAH box protein 9
  • EC 3.6.1
  • EC 3.6.1.15
  • EC 3.6.4.13
  • FLJ17406
  • leukophysin
  • LKP
  • NDH II
  • NDH2
  • NDHII
  • Nuclear DNA helicase II
  • RHA

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNA Helicase A Antibody (NB110-40579)(5)

Review for RNA Helicase A Antibody (NB110-40579) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-40579:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry RNA Helicase A NB110-40579
Enlarge
5
reviewed by:
Anonymous
ICC Human 03/03/2020
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman Cell lines,293T lysate
SpeciesHuman
LotA2

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Recent Reviews

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5
1
4
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2
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1
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Anonymous
03/03/2020
Application: ICC
Species: Human

Bioinformatics

Gene Symbol DHX9
Entrez
Uniprot