Biological Strategies: Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody [NB110-40579] - Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is ...read more
Immunohistochemistry: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB
Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 on HeLa whole cell lystate using NB110-40579.
Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by Western blot of immunoprecipitates. Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared ...read more
The immunogen recognized by this antibody maps to a region between residue 1220 and the C-terminus (residue 1270) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 9 using the numbering given in entry CAA71668.1 (GeneID 1660).
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Immunogen affinity purified
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Use in ICC/IF reported in scientific literature (PMID 25951186). Use in Knockdown reported in scientific literature (PMID: 30111593). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. This RNA Helicase A Antibody is validated for ICC/IF from a verified customer review.
Read 1 Review rated 5 using NB110-40579 in the following applications:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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