RUVBL2 Antibody

Images

 
Western Blot: RUVBL2 Antibody [NBP1-40354] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified ...read more
Immunohistochemistry: RUVBL2 Antibody [NBP1-40354] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RuvBL2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Proximity Ligation Assay: RUVBL2 Antibody [NBP1-40354] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: RUVBL2 Antibody [NBP1-40354] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DBC1 (NBP2-22294) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms), ...read more
Proximity Ligation Assay: RUVBL2 Antibody [NBP1-40354] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: RUVBL2 Antibody [NBP1-40354] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: RUVBL2 Antibody [NBP1-40354] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC5 (NB100-235) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: RUVBL2 Antibody [NBP1-40354] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RUVBL2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human RuvB-like 2 using the numbering given in entry NP_006657.1 (GeneID 10856).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RUVBL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 5-10 ug/mg
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-40354 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RUVBL2 Antibody

  • 48 kDa TATA box-binding protein-interacting protein
  • 51 kDa erythrocyte cytosolic protein
  • EC 3.6.1
  • EC 3.6.4.12,48 kDa TBP-interacting protein
  • ECP51TIP49B
  • erythrocyte cytosolic protein, 51-KD
  • INO80 complex subunit J
  • INO80JTAP54-beta
  • Repressing pontin 52
  • Reptin 52
  • REPTIN
  • Reptin52
  • RuvB (E coli homolog)-like 2
  • RuvB-like 2 (E. coli)
  • ruvB-like 2
  • RVB2
  • TBP-interacting protein, 48-KD
  • TIH2
  • TIP48ECP-51
  • TIP49b
  • TIP60-associated protein 54-beta

Background

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for RUVBL2 Antibody (NBP1-40354) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using NBP1-40354:
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Images Ratings Applications Species Date Details
Immunoprecipitation RUVBL2 NBP1-40354
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reviewed by:
Anonymous
IP Human 12/07/2017
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Summary

ApplicationImmunoprecipitation
Sample TestedOral cancer cell line
SpeciesHuman
LotA1

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Recent Reviews

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Anonymous
12/07/2017
Application: IP
Species: Human

Bioinformatics

Gene Symbol RUVBL2
Entrez