DDIT4 Antibody - BSA Free

DDIT4-Antibody-Western-Blot-NBP1-22966-img0005.jpg

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.

Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.

DDIT4-Antibody-Western-Blot-NBP1-22966-img0006.jpg

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - NBF-fixed asynchronous, cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L crossadsorbed Antibody DyLight®594 used at 1:100.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Eq, Pm, Pm-Or, Pm, Rb
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DDIT4 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human protein regulated in development and DNA damage response 1 (DNA-damage-inducible transcript 4) using the numbering given in entry NP_061931.1 (GeneID 54541).
• Predicted Species: Rat (100%), Canine (100%), Primate (100%), Equine (100%), Monkey (100%), Bovine (100%), Orangutan (100%), Rabbit (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDIT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500 to 1:2000
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:1000-1:4000
• Application Notes: Formaldehyde fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehydefixed cells.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25867045)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDIT4 Antibody - BSA Free

• Dig2
• DNA-damage-inducible transcript 4
• FLJ20500
• HIF-1 responsive RTP801 (RTP801)
• Protein regulated in development and DNA damage response 1
• REDD-1DNA damage-inducible transcript 4 protein
• REDD1HIF-1 responsive protein RTP801
• RTP801RP11-442H21.1

Background

REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDIT4 Antibody (NBP1-22966)(3)

Publications using NBP1-22966

• Hwang D, Baek S, Chang J et al. YAP promotes global mRNA translation to fuel oncogenic growth despite starvation. Experimental & molecular medicine 2024-10-01 [PMID: 39349825]
• Kuss-Duerkop SK, Wang J, Mena I et al. Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication. PLoS Pathog. 2017-09-01 [PMID: 28953980] (WB, Human)
• Potts MB, McMillan EA, Rosales TI et al. Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B Nat. Chem. Biol. 2015-04-13 [PMID: 25867045] (WB, Mouse)

Review for DDIT4 Antibody (NBP1-22966) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Natalie Erdmann ICC Human 05/24/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: hela cell
• Species: Human
• Lot: A1

Comments

• Comments: HeLa cells treated with 0.2% hypoxia or different doses CoCl2 overnight; fixed in 4% PFA 10 minutes (directly in hypoxia chamber or quickly after washing off CoCl2/media as half-life DDIT4 is 5-10 minutes); permeabilize 0.5% Triton X-100 5 minutes; dilute primary antibody 1/500 incubate O/N @4oC; wash 3X PBS; Alexa 488 donkey anti-rabbit F(ab”)2 secondary antibody 1/1000 1 hour at rt; wash 5X PBS; mount DAPI/Vectashield; images acquired on Olympus BX63 microscope with Bioview Allegro software.

Bioinformatics

• Gene Symbol: DDIT4
• Entrez:
  • 54541(Human)
• Uniprot:
  • Q9NX09(Human)