DDB1 Antibody

Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP1-33061] - Analysis of paraformaldehyde-fixed HeLa.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: DDB1 Antibody [NBP1-33061] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of DDB1 protein by 2. 5 ug of DDB1 antibody 7. 5% SDS-PAGE gel.

Western Blot: DDB1 Antibody [NBP1-33061] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:5000.

Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.

Western Blot: DDB1 Antibody [NBP1-33061] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Cal27 Xenograft , using DDB1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse prostate. DDB1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Rat brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Knockdown Validated: DDB1 Antibody [NBP1-33061] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody ).

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp
• Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DDB1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Chicken (98%), Xenopus (96%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:10000
• Theoretical MW: 127 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (96%), Xenopus Tropicalis (93%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for DDB1 Antibody

• damage-specific DNA binding protein 1 (127kD)
• damage-specific DNA binding protein 1, 127kDa
• Damage-specific DNA-binding protein 1
• DDB p127 Subunit
• DDB1
• DDBA
• DNA damage-binding protein 1
• DNA damage-binding protein a
• HBV X-associated protein 1
• UV-damaged DNA-binding factor
• UV-damaged DNA-binding protein 1
• UV-DDB 1
• UV-DDB1
• XAP1
• XAP-1
• Xeroderma pigmentosum group E-complementing protein
• XPCE
• XPE
• XPE-BF
• XPE-binding factor

Background

Damage specific DNA binding protein (DDB) functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DDB1 Antibody (NBP1-33061)(1)

Publication using NBP1-33061

• Goulielmaki E, Tsekrekou M, Batsiotos N et al. The Splicing Factor XAB2 interacts with ERCC1-XPF and XPG for RNA-loop processing during mammalian development bioRxiv Jul 21 2020 (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: DDB1
• Entrez:
  • 1642(Human)
• Uniprot:
  • Q16531()