Cullin 4a Antibody

Images

 
Western Blot: Cullin 4a Antibody [NB100-2267] - Detection of Human and Mouse Cul4a by WB (h&m) and Immunoprecipitation (h). Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; ...read more
Immunohistochemistry: Cullin 4a Antibody [NB100-2267] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Cul4a used at a dilution ...read more
Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267] - FFPE section of human breast tumor. Affinity purified rabbit anti-Cul4a used at a dilution of 1:500.
Proximity Ligation Assay: Cullin 4a Antibody [NB100-2267] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human CUL4a (NB100-2267). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Cullin 4a Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 759) of human Cullin 4A using the numbering given in entry NP_001008895.1 (GeneID 8451).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CUL4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
Cullin 4a Lysate (NBL1-09609)
Publications
Read Publications using
NB100-2267 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cullin 4a Antibody

  • CUL-4A
  • cullin 4A
  • cullin-4A

Background

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitinconjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cullin 4a Antibody (NB100-2267)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CUL4A
Entrez
Uniprot