Independent Antibodies: Western Blot: Cullin 4a Antibody [NB100-2267] - Detection of Human and Mouse Cul4a by WB (h&m) and Immunoprecipitation (h). Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for ...read more
Immunohistochemistry: Cullin 4a Antibody [NB100-2267] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Cul4a used at a dilution ...read more
Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267] - FFPE section of human breast tumor. Affinity purified rabbit anti-Cul4a used at a dilution of 1:500.
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitinconjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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