DCI Antibody

Western Blot: DCI Antibody [NBP2-16107] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: DCI Antibody [NBP2-16107] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DCI Antibody Summary

• Description: Novus Biologicals Rabbit DCI Antibody (NBP2-16107) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DCI. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ECI1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DCI Antibody

• acetylene-allene isomerase
• D2-enoyl-CoA isomerase
• D3
• DCI3,2-trans-enoyl-CoA isomerase3,2 trans-enoyl-CoA isomerase
• Delta(2)-enoyl-CoA isomerase
• Delta(3)
• delta3, delta2-enoyl-CoA isomerase
• dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase)
• dodecenoyl-CoA isomerase3,2-trans-enoyl-CoA isomerase, mitochondrial
• dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
• EC 5.3.3.8
• enoyl-CoA delta isomerase 13,2 trans-enoyl-Coenzyme A isomerase

Background

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ECI1
• Uniprot:
  • P42126(Human)