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Cathepsin A/Lysosomal Carboxypeptidase A Antibody

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Cathepsin A/Lysosomal Carboxypeptidase A was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Cathepsin A/Lysosomal Carboxypeptidase A Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Cathepsin A/Lysosomal Carboxypeptidase A Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin A/Lysosomal Carboxypeptidase A
Ala29-Tyr480
Accession # P10619
Specificity
Detects both pro and active forms of human and mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In Western blots, it recognizes all forms of recombinant human Cathepsin A: single chain (55 kDa), heavy chain (32 kDa), and light chain (20 kDa). Also in Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S and rhCathepsin X/Z/P is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSA
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1049 in the following applications:

Publications
Read Publications using
AF1049 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin A/Lysosomal Carboxypeptidase A Antibody

  • beta-galactosidase 2
  • beta-galactosidase protective protein
  • Carboxypeptidase C
  • Carboxypeptidase L
  • Cathepsin A
  • cathepsin ANGBE
  • CTSA
  • EC 3.4.16
  • EC 3.4.16.5
  • GLB2
  • GSL
  • Lysosomal Carboxypeptidase A
  • lysosomal protective protein
  • PPCA
  • PPGBprotective protein for beta-galactosidase (galactosialidosis)
  • Protective protein cathepsin A
  • Protective protein for beta-galactosidase

Background

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

  1. Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego.
  2. Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin A/Lysosomal Carboxypeptidase A Antibody (AF1049)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Dot Blot, ICC, IHC, Western Blot.


Filter By Application
Dot Blot
(1)
ICC
(1)
IHC
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF1049 Applications Species
GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion Redox Biology, 2021-12-05;48(0):102205. 2021-12-05 [PMID: 34891098] (Dot Blot, Human) Dot Blot Human
M Hohl, M Mayr, L Lang, AG Nickel, J Barallobre, X Yin, T Speer, SR Selejan, C Goettsch, K Erb, C Fecher-Tro, JC Reil, B Linz, S Ruf, T Hübschle, C Maack, M Böhm, T Sadowski, D Linz Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice J. Biol. Chem., 2020-07-09;0(0):. 2020-07-09 [PMID: 32647007] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
C Bonolo De, YX Zhu, N Sepetov, S Romanov, LA Bruins, CX Shi, CK Stein, JL Petit, AN Polito, ME Sharik, EW Meermeier, GJ Ahmann, ID Lopez Arme, J Kruse, PL Bergsagel, M Chesi, N Meurice, E Braggio, AK Stewart Identification of PIKfyve kinase as a target in multiple myeloma Haematologica, 2019-10-03;0(0):. 2019-10-03 [PMID: 31582538] (Western Blot, Human) Western Blot Human
Armstrong A, Mattsson N, Appelqvist H et al. Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer's Disease. Neuromolecular Med. 10/8/2013 [PMID: 24101586]
Reich M, Spindler KD, Burret M, Kalbacher H, Boehm BO, Burster T Cathepsin A is expressed in primary human antigen-presenting cells. Immunol. Lett., 2009-11-30;128(2):143-7. 2009-11-30 [PMID: 19954752] (ICC, Western Blot, Human) ICC, Western Blot Human

Review for Cathepsin A/Lysosomal Carboxypeptidase A Antibody (AF1049) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using AF1049:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Cathepsin A/Lysosomal Carboxypeptidase A AF1049
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4
reviewed by:
Verified Customer
Simple Western Human and Rat 08/30/2018
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Summary

ApplicationSimple Western
Sample TestedB103 lysate,SVG-A lysate
SpeciesHuman and Rat
LotHYI0215121

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Verified Customer
08/30/2018
Application: Simple Western
Species: Human and Rat

Bioinformatics

Gene Symbol CTSA
Uniprot