PECI Antibody - BSA Free

Western Blot: PECI Antibody [NBP1-89283] - Analysis using Anti-ECI2 antibody NBP1-89283 (A) shows similar pattern to independent antibody NBP2-48614 (B).

Immunocytochemistry/Immunofluorescence: PECI Antibody [NBP1-89283] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria & vesicles.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human kidney using Anti-ECI2 antibody NBP1-89283.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human skin shows very weak positivity in epidermal cells.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining in human kidney and skin tissues . Corresponding ECI2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human heart muscle, kidney, liver and testis using Anti-ECI2 antibody NBP1-89283 (A) shows similar protein distribution across tissues to independent antibody NBP2-48614 (B).

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human testis.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human heart muscle.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: PECI Antibody [NBP1-89283] - Staining of human liver using Anti-ECI2 antibody NBP1-89283.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PECI Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SLPKEAARQNYVDLVSSLSPSLESSSQVEPGTDRKSTGFETLVVTSEDGITKIMFNRPKKKNAINTEMYHEIMRALKAASKDDSIITVLTGNGDYYSSGNDLTNFTDIP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ECI2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PECI Protein (NBP1-89283PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PECI Antibody - BSA Free

• ACBD2
• acyl-Coenzyme A binding domain containing 2
• D2-enoyl-CoA isomerase
• delta(2)-enoyl-CoA isomerase
• Delta(3)
• Diazepam-binding inhibitor-related protein 1
• dJ1013A10.3
• Dodecenoyl-CoA isomerase
• DRS-1
• DRS1D3
• EC 5.3.3.8
• enoyl-CoA delta isomerase 2
• HCA88DBI-related protein 1
• Hepatocellular carcinoma-associated antigen 88
• KIAA0536
• PECI
• Peroxisomal 3,2-trans-enoyl-CoA isomerase
• peroxisomal D3
• Renal carcinoma antigen NY-REN-1

Background

PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PECI Antibody (NBP1-89283)(1)

Publication using NBP1-89283

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: ECI2