RelB Antibody

Western Blot: Rel B Antibody [NBP2-20123] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:10000.

Western Blot: Rabbit Polyclonal RelB Antibody [NBP2-20123] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. RRS1 Antibody (NBP2-49323) was used for primary antibody: 1:1000, 4C, overnight. Image from a verified customer review.

Western Blot: RelB Antibody [NBP2-20123] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (NBP2-20123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (NBP2-20123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

RelB antibody immunoprecipitates RelB protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 ug of RelB antibody (NBP2-20123) 7.5% SDS-PAGE The immunoprecipitated RelB protein was detected by RelB antibody (NBP2-20123) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

RelB antibody detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.RelB stained by RelB antibody (NBP2-20123) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 ug/ml for 3 h) and 5 ug of either normal rabbit IgG or anti-RelB antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-1betapromoter.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RelB Antibody Summary

• Description: Novus Biologicals Rabbit RelB Antibody (NBP2-20123) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-RelB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RELB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Application Notes: Chip assay-Assay dependent
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RelB Antibody

• IREL
• I-Rel
• nuclear factor of kappalight polypeptide gene enhancer in B-cells 3
• RelB
• v-rel reticuloendotheliosis viral oncogene homolog B

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RelB Antibody (NBP2-20123)(1)

Publication using NBP2-20123

• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (WB, Mouse)
  
  Details:
  Citation using the PE format of this antibody.

Review for RelB Antibody (NBP2-20123) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer WB 12/31/2023

Summary

• Application: Western Blot
• Lot: 43649

Bioinformatics

• Gene Symbol: RELB