RelB Antibody

Images

 
Western Blot: RelB Antibody [NBP2-20123] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: RelB Antibody [NBP2-20123] - Human breast carcinoma. RelB stained by RelB antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation: RelB Antibody [NBP2-20123] - Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 ug/ml for 3 h) and 5 ug of either normal rabbit IgG or anti-RelB antibody. ...read more
Western Blot: Rel B Antibody [NBP2-20123] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: RelB Antibody [NBP2-20123] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody diluted at 1:5000. The ...read more
Immunoprecipitation: RelB Antibody [NBP2-20123] - HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 ug of RelB antibody 7.5% SDS-PAGE The ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RelB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RELB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Chromatin Immunoprecipitation
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-20123 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for RelB Antibody

  • IREL
  • I-Rel
  • nuclear factor of kappalight polypeptide gene enhancer in B-cells 3
  • RelB
  • v-rel reticuloendotheliosis viral oncogene homolog B

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelB Antibody (NBP2-20123)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Blogs on RelB.

NFkB and p62 Both Activate and Regulate Inflammation
Nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) is a protein complex that regulates DNA transcription and is a critical regulator of cell survival. NFkB has long been known as a primer of inflammation, however researchers are...  Read full blog post.

RelA/NF-kB - A proinflammatory signaling pathway with roles in immunity and cancer
The inflammatory response consists of a complex network of signaling pathways that regulate a diverse set of cytokines, growth factors, adhesion molecules, and transcription factors (1). Of the proinflammatory signaling pathways the NF-kB family is...  Read full blog post.

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Bioinformatics

Gene Symbol RELB