DLL3 Antibody - BSA Free

Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: DLL3 Antibody [NBP2-24669] - Human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/mL.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DLL3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DLL3 Antibody - BSA Free (NBP2-24669) is a polyclonal antibody validated for use in IHC and WB. Anti-DLL3 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acid 100 to 150 of human DLL3 was used as immunogen for the antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLL3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry reported in scientific literature (PMID 31109352)
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 3 - 5 ug/mL
• Application Notes: IMG-6242A detects the expected approximately 23 kD band in mouse and rat skeletal muscle tissue lysate, but additional larger molecular weight bands may be seen.

Reactivity Notes

Cat (83%), Bovine (81%), Mouse, Rat (72%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for DLL3 Antibody - BSA Free

• delta (Drosophila)-like 3
• Delta3
• delta-like 3 (Drosophila)
• delta-like protein 3
• DLL3
• Drosophila Delta homolog 3
• Pudgy
• SCDO1
• SCDO1delta3

Background

The DLL3 gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostos

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DLL3 Antibody (NBP2-24669)(4)

Publications using NBP2-24669

• Bylsma LC, Pundole X, Ju CH et al. Systematic Literature Review of the Prevalence and Prognostic Value of Delta-Like Ligand 3 Protein Expression in Small Cell Lung Cancer Targeted oncology 2023-11-01 [PMID: 37930513]
• Chen W, Liu N, Shen S Et al. Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring Cell reports 2021-11-02 [PMID: 34731622] (WB, Mouse)
• Kuempers C, Jagomast T, Krupar R Et al. Delta-Like Protein 3 Expression in Paired Chemonaive and Chemorelapsed Small Cell Lung Cancer Samples Frontiers in medicine 2021-10-08 [PMID: 34692726]
• Toshima S, Kurihara Y, Wang EHC et al. Comparison of four DLL3 antibodies performance in high grade neuroendocrine lung tumor samples and cell cultures Diagn Pathol 2019-05-20 [PMID: 31109352] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: DLL3
• Uniprot:
  • Q9NYJ7(Human)