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DLL3 Antibody

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Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. ...read more
Immunohistochemistry-Paraffin: DLL3 Antibody [NBP2-24669] - Human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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DLL3 Antibody Summary

Immunogen
A portion of amino acid 100 to 150 of human DLL3 was used as immunogen for the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLL3
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 3 - 5 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
Application Notes
IMG-6242A detects the expected approximately 23 kD band in mouse and rat skeletal muscle tissue lysate, but additional larger molecular weight bands may be seen. Use in Immunohistochemistry reported in scientific literature (PMID: 31109352).
Publications
Read Publication using
NBP2-24669 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Cat (83%), Bovine (81%), Mouse, Rat (72%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for DLL3 Antibody

  • delta (Drosophila)-like 3
  • Delta3
  • delta-like 3 (Drosophila)
  • delta-like protein 3
  • DLL3
  • Drosophila Delta homolog 3
  • Pudgy
  • SCDO1
  • SCDO1delta3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Mouse splenocytes were stained with Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
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Western Blot: MESP2 Antibody (1B3F9) [NBP2-37424] - Analysis using MESP2 mAb against HEK293 (1) and MESP2 (AA: 37-94)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: MESP2 Antibody (1B3F9) [NBP2-37424] - Analysis using MESP2 mAb against human MESP2 recombinant protein. (Expected MW is 31.4 kDa)
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Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).
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HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
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DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Notch-4 Antibody
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NBP2-24716
Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.
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Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human uterine cervix shows strong positivity in the extracellular matrix.
Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human stomach shows moderate positivity in extracellular matrix.
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(OTI4H1)
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Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.
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DLL3 Antibody
NBP2-24669
DLL3 Antibody
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
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Publications for DLL3 Antibody (NBP2-24669)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol DLL3
Uniprot