DCI Antibody (SR2403)

Immunohistochemistry-Paraffin: DCI Antibody (SR2403) [NBP3-27695] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western Blot: DCI Antibody (SR2403) [NBP3-27695] - Western blot analysis of DCI in K562, rat Brain, C6, 3T3, Hela lysates

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SR2403
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DCI Antibody (SR2403) Summary

• Description: Novus Biologicals Rabbit DCI Antibody (SR2403) (NBP3-27695) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human DCI (Uniprot #: P42126)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ECI1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for DCI Antibody (SR2403)

• acetylene-allene isomerase
• D2-enoyl-CoA isomerase
• D3
• DCI3,2-trans-enoyl-CoA isomerase3,2 trans-enoyl-CoA isomerase
• Delta(2)-enoyl-CoA isomerase
• Delta(3)
• delta3, delta2-enoyl-CoA isomerase
• dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase)
• dodecenoyl-CoA isomerase3,2-trans-enoyl-CoA isomerase, mitochondrial
• dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
• EC 5.3.3.8
• enoyl-CoA delta isomerase 13,2 trans-enoyl-Coenzyme A isomerase

Background

The DCI gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising durin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECI1