DAX1/NR0B1 Antibody (JU33-05)

Western Blot: DAX1/NR0B1 Antibody (JU33-05) [NBP2-75462] - Analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody (JU33-05) [NBP2-75462] - Staining NR0B1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: DAX1/NR0B1 Antibody (JU33-05) [NBP2-75462] - Analysis of A549 cells with NR0B1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody (JU33-05) [NBP2-75462] - Staining NR0B1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody (JU33-05) [NBP2-75462] - Staining NR0B1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

DAX1/NR0B1 Antibody (JU33-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human DAX1/NR0B1 aa 1-50 / 470. (SwissProt: P51843 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR0B1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DAX1/NR0B1 Antibody (JU33-05)

• AHC
• AHCDSS
• AHCH
• AHX
• DAX1
• DAX1DAX-1
• dosage-sensitive sex reversal
• DSS-AHC critical region on the X chromosome protein 1
• GTD
• HHG
• NR0B1
• NROB1
• nuclear hormone receptor
• Nuclear receptor DAX-1
• nuclear receptor subfamily 0 group B member 1
• nuclear receptor subfamily 0, group B, member 1

Background

NR0B1 encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR0B1