DAD1 Antibody

Images

 
Western Blot: DAD1 Antibody [NBP1-77197] - Analysis of DAD1 in HepG2 cell lysates with anti-DAD1 using this antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: DAD1 Antibody [NBP1-77197] - Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: DAD1 Antibody [NBP1-77197] - HepG2 cells with DAD1 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DAD1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1.
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAD1
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 mg/ml. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE.
Control Peptide
DAD1 Peptide (NBP1-77197PEP)

Reactivity Notes

Chicken: (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for DAD1 Antibody

  • DAD-1
  • defender against cell death 1EC 2.4.1.119
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
  • Oligosaccharyl transferase subunit DAD1
  • oligosaccharyltransferase 2 homolog
  • OST2

Background

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46439
Species: Hu
Applications: WB, IHC
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-01294
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
H00023263-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-80835
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84463
Species: Hu
Applications: IHC, IHC-P
NBP1-84297
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84466
Species: Hu
Applications: WB, IHC, IHC-P
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NB100-56146
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-31660
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-40681
Species: Hu, Mu
Applications: WB, IHC-P, IP
NB100-56565
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
H00004831-M06
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for DAD1 Antibody (NBP1-77197) (0)

There are no publications for DAD1 Antibody (NBP1-77197).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DAD1 Antibody (NBP1-77197) (0)

There are no reviews for DAD1 Antibody (NBP1-77197). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DAD1 Antibody (NBP1-77197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DAD1 Products

Bioinformatics Tool for DAD1 Antibody (NBP1-77197)

Discover related pathways, diseases and genes to DAD1 Antibody (NBP1-77197). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DAD1 Antibody (NBP1-77197)

Discover more about diseases related to DAD1 Antibody (NBP1-77197).
 

Pathways for DAD1 Antibody (NBP1-77197)

View related products by pathway.

PTMs for DAD1 Antibody (NBP1-77197)

Learn more about PTMs related to DAD1 Antibody (NBP1-77197).
 

Research Areas for DAD1 Antibody (NBP1-77197)

Find related products by research area.

Blogs on DAD1

There are no specific blogs for DAD1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DAD1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DAD1
Uniprot