DAD1 Antibody - BSA Free

Western Blot: DAD1 Antibody [NBP1-77197] - Analysis of DAD1 in HepG2 cell lysates with anti-DAD1 using this antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: DAD1 Antibody [NBP1-77197] - Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.

Immunocytochemistry/Immunofluorescence: DAD1 Antibody [NBP1-77197] - HepG2 cells with DAD1 antibody at 10 ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DAD1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1. Amino Acid Squence: CLRIQINPQNKAD
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DAD1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE.
• Control Peptide:

  DAD1 Peptide (NBP1-77197PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for DAD1 Antibody - BSA Free

• DAD-1
• defender against cell death 1EC 2.4.1.119
• dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• Oligosaccharyl transferase subunit DAD1
• oligosaccharyltransferase 2 homolog
• OST2

Background

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAD1 Antibody (NBP1-77197)(1)

Publication using NBP1-77197

• Schnormeier AK, Pommerenke C, Kaufmann M et al. Genomic deregulation of PRMT5 supports growth and stress tolerance in chronic lymphocytic leukemia Sci Rep 2020-06-17 [PMID: 32555249] (WB, Human)

Bioinformatics

• Gene Symbol: DAD1
• Uniprot:
  • AAH09798(Human)