Ribophorin II Antibody

Western Blot: Ribophorin II Antibody [NBP1-80835] - Analysis in human cell line SK-BR-3.

Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining in human epididymis and skeletal muscle tissues using NBP1-80835 antibody. Corresponding RPN2 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ribophorin II Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFD
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Ribophorin II Protein (NBP1-80835PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Ribophorin II Antibody

• RIBIIREC 2.4.1.119
• ribophorin IIDolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
• ribophorin-2
• RPNII
• RPN-IIdolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
• SWP1

Background

Ribophorin II encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ribophorin II Antibody (NBP1-80835)(1)

Publication using NBP1-80835

• Lyu J, Imachi H, Fukunaga K et al. Exendin-4 Increases Scavenger Receptor Class BI Expression via Activation of AMPK/FoxO1 in Human Vascular Endothelial Cells Current issues in molecular biology 2022-11-03 [PMID: 36354682] (WB, Human)

Bioinformatics

• Gene Symbol: RPN2
• Entrez:
  • 6185(Human)
• Uniprot:
  • P04844(Human)