![Western Blot: Ribophorin II Antibody [NBP1-80835] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Western-Blot-NBP1-80835-img0019.jpg "Western Blot: Ribophorin II Antibody [NBP1-80835] - Analysis in human cell line SK-BR-3.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Ribophorin II Antibody - BSA Free (NBP1-80835) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Ribophorin II Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFD |
| Predicted Species | Mouse (98%), Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RPN2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-80835 | Applications | Species |
|---|---|---|
| Lyu J, Imachi H, Fukunaga K et al. Exendin-4 Increases Scavenger Receptor Class BI Expression via Activation of AMPK/FoxO1 in Human Vascular Endothelial Cells Current issues in molecular biology 2022-11-03 [PMID: 36354682] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80835-img0007.jpg "Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0018.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0013.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0014.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0015.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0016.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining in human epididymis and skeletal muscle tissues using NBP1-80835 antibody. Corresponding RPN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0017.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining in human epididymis and skeletal muscle tissues using NBP1-80835 antibody. Corresponding RPN2 RNA-seq data are presented for the same tissues.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")