D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate.

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI.

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein.

• Reactivity: Hu, Bv, Ca, Ch, Eq
• Applications: WB, Flow, ICC/IF
• Clone: 10D774
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free Summary

• Description: Novus Biologicals Mouse D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free (NB100-56556) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. Anti-D4-GDI/RhoGDI2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen.
• Predicted Species: Equine (100%), Chicken (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGDIB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 25623267)
  • Western Blot 1-2 ug/ml
• Application Notes: A 28 kDa band is observed.
• Control:

  D4-GDI/RhoGDI2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.02% Gelatin
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

• ARHGDIB Rho GDP dissociation inhibitor (GDI) beta
• D4
• GDIA2
• GDID4
• LYGDI
• Ly-GDI
• RAP1GN1
• RhoGDI2

Background

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for D4-GDI/RhoGDI2 Antibody (NB100-56556)(3)

Publications using NB100-56556

• Barbati C, Alessandri C, Vomero M et al. Autoantibodies specific to D4GDI modulate Rho GTPase mediated cytoskeleton remodeling and induce autophagy in T lymphocytes J. Autoimmun. 2015-01-23 [PMID: 25623267] (ICC/IF, FLOW, WB, Human)
  
  Details:
  D4-GDI/RhoGDI2 /mAb anti D4GDI antibody used for ICC-IF and FLOW in experiments involving T lymphocytes from patients with Systemic Lupus Erythematosus/SLE disease. For FLOW naive subse/CD45RA+CD62L+, central memory subset/CD45RA-CD62L+, and effector memory subset/CD45RA+CD62L- and CD45RA-CD62L- cells were immunostained with D4GDI antibody, human IgG purified from sera of patients, and IVIG/intravenous immunoglobulin (negative control) - each used at 1 ug /1 x 10(6) cells concentration followed by detection via FITC-conjugated secondary antibodies and 50,000 events/sample on FACSCalibur cytometer (Fig. 2A, Fig. 3C, Supplemental Fig 1). Antibody also used for WB and FLOW on Jurkat cells that were treated or not with siRNA-D4GDI for knockdown of D4GDI protein (Fig. 5). For ICC-IF, peripheral blood T lymphocytes were fixed in 4% PFA-PBS for 30 minutes at 4C, blocked in 2% BSA-PBS-5% glycerol-0.2% Tween 20 for 1 hour at 4C, incubated with primary antibody at 0.1 ug/uL in PBS-1% BSA for 1 hour at 4C followed by FITC conjugated secondary for 30 minutes at 4C and analysis using Olympus U RFL microscope (Fig. 2B, 3D).
• Brown MG, Huang YY, Marshall JS et al. Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection of human mast cells. J Leukoc Biol. 2009-01-01 [PMID: 18809735] (WB, Human)
  
  Details:
  WB (human KU812 cells), Fig. 8.
• Lee SH, Lee JW, Kim HS et al. Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer APMIS 2003-05-01 [PMID: 12887511]
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: ARHGDIB