D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate. Image from the standard format of this antibody.

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI. Image from the standard format of this antibody.

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: 10D774
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free Summary

• Immunogen: A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGDIB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 25623267)
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.02% gelatin.
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free

• ARHGDIB Rho GDP dissociation inhibitor (GDI) beta
• D4
• GDIA2
• GDID4
• LYGDI
• Ly-GDI
• RAP1GN1
• RhoGDI2

Background

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGDIB