Immunocytochemistry/ Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
Western Blot: 5-Lipoxygenase Antibody [NB110-58748] - Detection of LOX5 in human peripheral leukocytes using NB110-58748 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).
Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using ...read more
Genetic Strategies: Knockdown Validated: 5-Lipoxygenase Antibody [NB110-58748] - Knockdown of 5-Lipoxygenase inhibits TNF-alpha-induced IL-6 and MCP-1 release in human synovial fibroblasts.Total protein extracts ...read more
This 5 Lipoxygenase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at approx. 80 kDa. There may be some non-specific bands at non-interfering lower molecular weights. In ICC/IF cytoplasmic staining was observed in Hela cells.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for 5-Lipoxygenase Antibody - BSA Free
arachidonic 5-lipoxygenase alpha-10 isoform
arachidonic 5-lipoxygenase delta-10-13 isoform
arachidonic 5-lipoxygenase delta-13 isoform
arachidonic 5-lipoxygenase delta-p10 isoform
arachidonic acid 5-lipoxygenase
leukotriene A4 synthase
5-lipoxygenase (5LO) is a pro-inflammatory enzyme widely expressed in CNS where its level increases in an age-dependent manner and its activity is regulated by the presence/availability of FLAP (5LO activating protein) that form a functional complex with 5LO to catalyze arachidonic acid oxidation to produce lipid molecules with complex signaling properties such as leukotrienes. Reaction catalyzed by 5LO is considered as first step in the leukotriene biosynthesis and it is highly critical to inflammatory processes. Moreover, knockout of 5LO as well as FLAP has been shown to result in anxiety-like behavior in mice and 5LO is emerging as a new active player in Alzheimer's disease (AD) pathogenesis. 5LO levels are significantly increased in AD postmortem brain tissues and its genetic absence or pharmacological blockade both result in a significant reduction in the abeta levels as well as deposition in a transgenic mouse model of AD-like amyloidosis (i.e., Tg2576) mice.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.