5-Lipoxygenase Antibody (NB110-58748)

Images

 
Western Blot: 5-Lipoxygenase Antibody [NB110-58748] - Detection of LOX5 in human peripheral leukocytes using NB110-58748 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).
Immunocytochemistry/ Immunofluorescence: 5-Lipoxygenase Antibody [NB110-58748] - 5-Lipoxygenase antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

5-Lipoxygenase Antibody Summary

Immunogen
A synthetic peptide made to a region within residues 100-200 of human 5-Lipoxygenase. [Swiss-Prot# P09917]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALOX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 50 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2000
Application Notes
This 5 Lipoxygenase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at approx. 80 kDa. There may be some non-specific bands at non-interfering lower molecular weights. In ICC/IF cytoplasmic staining was observed in Hela cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Blood Leukocyte Lysate (NB820-59174)
Control Peptide
5-Lipoxygenase Peptide (NB110-58748PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB110-58748 in the following applications:

Publications
Read Publication using NB110-58748.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-Lipoxygenase Antibody

  • 5Lipoxygenase
  • 5-Lipoxygenase
  • 5-LO
  • 5-LOX
  • 5LPG
  • ALOX5
  • arachidonate 5-lipoxygenase
  • arachidonic 5-lipoxygenase alpha-10 isoform
  • arachidonic 5-lipoxygenase delta-10-13 isoform
  • arachidonic 5-lipoxygenase delta-13 isoform
  • arachidonic 5-lipoxygenase delta-p10 isoform
  • arachidonic acid 5-lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.34
  • leukotriene A4 synthase
  • LOG5
  • LOX5A
  • MGC163204

Background

5-lipoxygenase (5LO) is a pro-inflammatory enzyme widely expressed in CNS where its level increases in an age-dependent manner and its activity is regulated by the presence/availability of FLAP (5LO activating protein) that form a functional complex with 5LO to catalyze arachidonic acid oxidation to produce lipid molecules with complex signaling properties such as leukotrienes. Reaction catalyzed by 5LO is considered as first step in the leukotriene biosynthesis and it is highly critical to inflammatory processes. Moreover, knockout of 5LO as well as FLAP has been shown to result in anxiety-like behavior in mice and 5LO is emerging as a new active player in Alzheimer's disease (AD) pathogenesis. 5LO levels are significantly increased in AD postmortem brain tissues and its genetic absence or pharmacological blockade both result in a significant reduction in the abeta levels as well as deposition in a transgenic mouse model of AD-like amyloidosis (i.e., Tg2576) mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5-Lipoxygenase Antibody (NB110-58748)(1)

Reviews for 5-Lipoxygenase Antibody (NB110-58748) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: WB.

Reviews using NB110-58748:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Bishuang Cai
WB Human 06/13/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage
SpeciesHuman
Lota1
 
reviewed by:
Anonymous
WB Human 05/24/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage
SpeciesHuman
Lota1

Product General Protocols

View specific protocols for 5-Lipoxygenase Antibody (NB110-58748): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-Lipoxygenase Antibody (NB110-58748) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-58748AF405
Alexa Fluor 488 Labeled NB110-58748AF488
Alexa Fluor 647 Labeled NB110-58748AF647
Alexa Fluor 700 Labeled NB110-58748AF700
Biotin Labeled NB110-58748B
DyLight 405 Labeled NB110-58748V
DyLight 488 Labeled NB110-58748G
DyLight 550 Labeled NB110-58748R
DyLight 650 Labeled NB110-58748C
FITC Labeled NB110-58748F
HRP Labeled NB110-58748H

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Bishuang Cai
06/13/2017
Application: WB
Species: Human

 
Anonymous
05/24/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol ALOX5
Entrez

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