Cytokeratin 18 Antibody

Images

 
Western Blot: Cytokeratin 18 Antibody [NBP1-90353] - Analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-KRT18 antibody. Corresponding KRT18 RNA-seq data are presented for the same cell lines. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody [NBP1-90353] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.
Western Blot: Cytokeratin 18 Antibody [NBP1-90353] - Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line EFO-21. Lane 4: Human cell line A-431

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
       

Orthogonal Strategies

 

Order Details

Cytokeratin 18 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:IEESTTVVTTQSTEVGAAKAMLTELTRTVQFLEINLDSMRNLKASLENSLREVVARYALQMEQLNGILLHLESELAQTWAEGQCQAQEYQALLNIKVKLEAAIATYRRLLEDG
Marker
Epithelial Marker
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cytokeratin 18 Protein (NBP1-90353PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 18 Antibody

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cytokeratin 18 Antibody (NBP1-90353)

Discover related pathways, diseases and genes to Cytokeratin 18 Antibody (NBP1-90353). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Entrez