Cytokeratin 18 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human small intestine shows moderate cytoplasmic and membrane positivity in glandular cells.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human kidney shows moderate cytoplasmic and membrane positivity in cells in tubules.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human placenta shows moderate cytoplasmic and membrane positivity in trophoblastic cells.

Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-KRT18 antibody. Corresponding KRT18 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 18 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cytokeratin 18 Antibody - BSA Free (NBP1-90353) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IEESTTVVTTQSTEVGAAKAMLTELTRTVQFLEINLDSMRNLKASLENSLREVVARYALQMEQLNGILLHLESELAQTWAEGQCQAQEYQALLNIKVKLEAAIATYRRLLEDG
• Marker: Epithelial Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT18
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Cytokeratin 18 Protein (NBP1-90353PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cytokeratin 18 Antibody - BSA Free

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18