Genetic Strategies: Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted ...read more
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE gel, diluted at 1:10000.
54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Expected cross reactivity based on sequence homology: Rat (87%), Porcine (83%), Bovine (84%), Canine (89%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
1XPBS, 20% Glycerol (pH7).
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Cytokeratin 8 Antibody
keratin, type II cytoskeletal 8
Type-II keratin Kb8
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Cytokeratin 8 Antibody (NBP2-16094)(3)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.