Cytokeratin 8 Antibody

Images

 
Genetic Strategies: Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT8 protein stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE gel, diluted at 1:10000.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Cytokeratin 8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.
Localization
Cytoplasm
Marker
Epithelial Cell Marker
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 8 Lysate (NBP2-64945)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-16094 in the following applications:

Publications
Read Publications using
NBP2-16094 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (87%), Porcine (83%), Bovine (84%), Canine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 8 Antibody

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • Keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cytokeratin 8 Antibody (NBP2-16094)(3)

Review for Cytokeratin 8 Antibody (NBP2-16094) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-16094:
Filter by Applications
ICC/IF
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence Cytokeratin 8 NBP2-16094
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reviewed by:
Alexandra Ambrico
ICC/IF Mouse 10/06/2016
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedmouse prostate
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 8 Antibody (NBP2-16094) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cytokeratin 8 Antibody (NBP2-16094)

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Diseases for Cytokeratin 8 Antibody (NBP2-16094)

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Research Areas for Cytokeratin 8 Antibody (NBP2-16094)

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Blogs on Cytokeratin 8.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Recent Reviews

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1
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2
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1
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Alexandra Ambrico
10/06/2016
Application: ICC/IF
Species: Mouse

Bioinformatics

Gene Symbol KRT8
Entrez
Uniprot