HOXB2 Antibody (PCRP-HOXB2-1F2)

Images

 
Immunocytochemistry/ Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse ...read more
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in ...read more
Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for ...read more
Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HOXB2 Antibody (PCRP-HOXB2-1F2).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P, IP, PA
Clone
PCRP-HOXB2-1F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HOXB2 Antibody (PCRP-HOXB2-1F2) Summary

Immunogen
Recombinant fragment (aa114-220) of human HOXB2 (Uniprot: P14652)
Localization
Nucleus, Nucleoplasm
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HOXB2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14063)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for HOXB2 Antibody (PCRP-HOXB2-1F2)

  • homeo box 2H
  • homeo box B2
  • homeobox B2
  • Homeobox protein Hox-2.8
  • Homeobox protein Hox-2H
  • homeobox protein Hox-B2
  • HOX2
  • HOX2HHox-2.8
  • K8 home protein
  • K8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HOXB2 Antibody (NBP3-13937) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HOXB2
Uniprot