Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

Western Blot: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining in 13 year old testis. Staining in Leydig cell. Image from a verified customer review.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining in macaque Leydig cells. Image from a verified customer review.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human breast tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human CYP17A1(NP_000093) peoduced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP17A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 57.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

• CPT7
• CYP17
• CYP17A1
• CYPXVII
• Cytochrome P450 17A1
• cytochrome p450 XVIIA1
• cytochrome P450, family 17, subfamily A, polypeptide 1
• cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia
• Cytochrome P450c17
• Cytochrome P450-C17
• EC 1.14.14.19
• EC 1.14.14.32
• EC 1.14.99
• P450C17
• P450C17CYPXVII
• S17AH

Background

The CYP17A1 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The CYP17A1 protein localizes to the endoplasmic reticulum. CYP17A1 has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in the CYP17A1 gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. (provided by RefSeq, Jul 2008)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP17A1