COMT Antibody

Images

 
Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by ...read more
Flow Cytometry: COMT Antibody [NB100-1780] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Western Blot: COMT Antibody [NB100-1780] - Staining of Human Liver (A) with antibody at 2 ug/mL and Ovary (B) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

COMT Antibody Summary

Immunogen
Peptide with sequence GDTKEQRILNHVLQC corresponding to N-Terminus according to NP_000745.1, NP_001128633.1, NP_001128634.1, NP_009294.1.
Specificity
This antibody is expected to recognize both reported isoforms. Variants (NP_000745.1; NP_001128633.1; NP_001128634.1) encode the same isoform.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COMT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 2 ug/mL
Control
Human Testis Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
COMT Protein (NB100-1780PEP)
Publications
Read Publications using
NB100-1780 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 19770404).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COMT Antibody

  • catechol O-methyltransferase
  • catechol-O-methyltransferase
  • COMT
  • EC 2.1.1.6

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COMT Antibody (NB100-1780)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COMT
Entrez
Uniprot