CREM Antibody (3B5)

Western Blot: CREM Antibody (3B5) [H00001390-M02] - CREM monoclonal antibody (M02), clone 3B5. Analysis of CREM expression in human liver.

Immunocytochemistry/Immunofluorescence: CREM Antibody (3B5) [H00001390-M02] - UTGB1, primary glioblastoma cell line, expressing the EWSR1-CREM fusion, stained using CREM antibody at 1:200. Secondary Antibody: Alexa Fluor 568 conjugate. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: CREM Antibody (3B5) [H00001390-M02] - Analysis of monoclonal antibody to CREM on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: CREM Antibody (3B5) [H00001390-M02] - Detection limit for recombinant GST tagged CREM is 0.03 ng/ml as a capture antibody.

Knockdown Validated: CREM Antibody (3B5) [H00001390-M02] - UTGB1, primary glioblastoma cell line, expressing the EWSR1-CREM fusion, treated with non-target siRNA and different concentrations of CREM siRNA. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, S-ELISA, KD
• Clone: 3B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CREM Antibody (3B5) Summary

• Immunogen: CREM (NP_853549, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY
• Specificity: CREM (3B5)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CREM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CREM Antibody (3B5)

• cAMP response element modulator
• cAMP responsive element modulator
• cAMP-responsive element modulator
• hCREM 2alpha-b protein
• hCREM 2beta-a protein
• hCREM-2
• ICER
• inducible cAMP early repressor ICER
• Inducible cAMP early repressor
• MGC111110
• MGC17881
• MGC41893

Background

This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for CREM Antibody (H00001390-M02) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 08/30/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: human glioblastoma,Primary Glioblastoma cell line UTGB1
• Species: Human
• Lot: 10061-3B5

reviewed by: Verified Customer WB Human 08/30/2018

Summary

• Application: Western Blot
• Sample Tested: Primary Glioblastoma cell line UTGB1
• Species: Human
• Lot: 10061-3B5

Bioinformatics

• Gene Symbol: CREM
• Entrez:
  • 1390(Human)
• OMIM:
  • 123812(Human)
• Uniprot:
  • NP_853549(Human)