Cyclin B2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody [NBP1-89657] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Staining in human bone marrow and cerebral cortex tissues using anti-CCNB2 antibody. Corresponding CCNB2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Staining of human bone marrow shows high expression.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Staining of human cerebral cortex shows low expression as expected.

Analysis in human cell line A-431.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cyclin B2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TVSSDLENIDTGVNSKVKSHVTIRRTVLEEIGNRVTTRAAQVAKKAQNTKVPVQPTKTTNVNKQLKPTASVKPVQMEKLAPKGPSPTPEDVSMKEENLCQAFSDALLCKIEDIDNEDWENPQLC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCNB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Cyclin B2 Protein (NBP1-89657PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%). Reactivity reported in scientific literature (PMID: 23282137)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cyclin B2 Antibody - BSA Free

• CCNB2
• Cyclin B2
• G2/mitotic-specific cyclin-B2
• HsT17299

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin B2 Antibody (NBP1-89657)(1)

Publication using NBP1-89657

• Shubbar E, Kovacs A, Hajizadeh S et al. Elevated cyclin B2 expression in invasive breast carcinoma is associated with unfavorable clinical outcome. BMC Cancer 2013-01-02 [PMID: 23282137]

Bioinformatics

• Gene Symbol: CCNB2