Histone H1 Antibody (HH1/957)

Images

 
Immunocytochemistry/ Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).
Immunocytochemistry/ Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image ...read more
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clone
HH1/957
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Histone H1 Antibody (HH1/957) Summary

Immunogen
Recombinant full-length human Histone H1 protein
Localization
Nuclear
Marker
Nuclear Marker
Specificity
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HIST1H1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 3q21-q22; 6q21-q22; 22q13.1
Molecular weight of antigen: ~30kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-45184 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Histone H1 Antibody (HH1/957)

  • H1 histone family, member 1
  • H1.1
  • H1A
  • H1F1HIST1
  • histone 1, H1a
  • histone cluster 1, H1a
  • histone H1.1
  • MGC126642
  • MGC138345

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H1 Antibody (NBP2-45184) (0)

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Review for Histone H1 Antibody (NBP2-45184) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-45184:
Filter by Applications
IF
(1)
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Filter by Species
Human
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Images Ratings Applications Species Date Details
Immunofluorescence Histone H1 NBP2-45184
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reviewed by:
Anonymous
IF Human 03/23/2018
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Summary

ApplicationImmunofluorescence
Sample TestedCell culture
SpeciesHuman
Lot3005-3P170404

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Histone H1 Antibody (NBP2-45184) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47754AF350
Alexa Fluor 405 NBP2-47754AF405
Alexa Fluor 488 NBP2-47754AF488
Alexa Fluor 532 NBP2-47754AF532
Alexa Fluor 594 NBP2-47754AF594
Alexa Fluor 647 NBP2-47754AF647
Alexa Fluor 700 NBP2-47754AF700
Alexa Fluor 750 NBP2-47754AF750
Allophycocyanin NBP2-47754APC
Biotin NBP2-47754B
DyLight 350 NBP2-47754UV
DyLight 405 NBP2-47754V
DyLight 488 NBP2-47754G
DyLight 550 NBP2-47754R
DyLight 594 NBP2-47754DL594
DyLight 650 NBP2-47754C
DyLight 680 NBP2-47754FR
DyLight 755 NBP2-47754IR
FITC NBP2-47754F
HRP NBP2-47754H
Janelia Fluor 549 NBP2-47754JF549
Janelia Fluor 646 NBP2-47754JF646
PE NBP2-47754PE
PerCP NBP2-47754PCP

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Bioinformatics Tool for Histone H1 Antibody (NBP2-45184)

Discover related pathways, diseases and genes to Histone H1 Antibody (NBP2-45184). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Histone H1 Antibody (NBP2-45184)

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Pathways for Histone H1 Antibody (NBP2-45184)

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Blogs on Histone H1.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

Recombinant Monoclonal Antibodies

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Recent Reviews

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Anonymous
03/23/2018
Application: IF
Species: Human

Bioinformatics

Gene Symbol HIST1H1A
Uniprot
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