![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunofluorescence-NBP2-45184-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P |
| Clone | HH1/957 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Recombinant full-length human Histone H1 protein |
| Localization | Nuclear |
| Marker | Nuclear Marker |
| Specificity | Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HIST1H1A |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Hu-chromosome location: 3q21-q22; 6q21-q22; 22q13.1 Molecular weight of antigen: ~30kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
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| Reviewed Applications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Anonymous |
IF | Human | 03/23/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-47754AF350 | |
| Alexa Fluor 405 | NBP2-47754AF405 | |
| Alexa Fluor 488 | NBP2-47754AF488 | |
| Alexa Fluor 532 | NBP2-47754AF532 | |
| Alexa Fluor 594 | NBP2-47754AF594 | |
| Alexa Fluor 647 | NBP2-47754AF647 | |
| Alexa Fluor 700 | NBP2-47754AF700 | |
| Alexa Fluor 750 | NBP2-47754AF750 | |
| Allophycocyanin | NBP2-47754APC | |
| Biotin | NBP2-47754B | |
| DyLight 350 | NBP2-47754UV | |
| DyLight 405 | NBP2-47754V | |
| DyLight 488 | NBP2-47754G | |
| DyLight 550 | NBP2-47754R | |
| DyLight 594 | NBP2-47754DL594 | |
| DyLight 650 | NBP2-47754C | |
| DyLight 680 | NBP2-47754FR | |
| DyLight 755 | NBP2-47754IR | |
| FITC | NBP2-47754F | |
| HRP | NBP2-47754H | |
| Janelia Fluor 549 | NBP2-47754JF549 | |
| Janelia Fluor 646 | NBP2-47754JF646 | |
| PE | NBP2-47754PE | |
| PerCP | NBP2-47754PCP |
Diseases for Histone H1 Antibody (NBP2-45184)Discover more about diseases related to Histone H1 Antibody (NBP2-45184).
| Pathways for Histone H1 Antibody (NBP2-45184)View related products by pathway.
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Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post. |
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![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize2/Histone H1 Antibody (HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0008.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunocytochemistry-Immunofluorescence-NBP2-45184-img0005.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0001.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0002.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0004.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0003.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0007.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).")
![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize2/Histone-H1.2-Antibody-(JU43-48)-Western-Blot-NBP2-75932-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H1.2-Antibody-(JU43-48)-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0007.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H1.2-Antibody-(JU43-48)-Immunohistochemistry-Paraffin-NBP2-75932-img0008.jpg)
![Western Blot: Histone H1.5 Overexpression Lysate (Adult Normal) [NBL1-11552] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H1.5.](http://images.novusbio.com/fullsize/Histone H1.5 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-11552-img0002.jpg)
![Western Blot: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on different cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution. Positive control: Lane 1: Untreated CRC whole cell lysates Lane2: CRC cells treated with](http://images.novusbio.com/fullsize2/Histone H1.4 [p Thr17] Antibody (SR38-03)-Western Blot-NBP2-67313-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Histone-H1.4-[p-Thr17]-Antibody-(SR38-03)-Immunocytochemistry-Immunofluorescence-NBP2-67313-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H1.4-[p-Thr17]-Antibody-(SR38-03)-Immunohistochemistry-Paraffin-NBP2-67313-img0003.jpg)
![Western Blot: Histone H1.3 Overexpression Lysate (Adult Normal) [NBL1-11554] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H1.3.](http://images.novusbio.com/fullsize/Histone H1.3 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-11554-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human testis.](http://images.novusbio.com/fullsize2/Histone-H1T-Antibody-Immunohistochemistry-Paraffin-NBP2-56042-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human testis shows nuclear positivity in ductus seminiferous.](http://images.novusbio.com/fullsize2/Histone-H1T-Antibody-Immunohistochemistry-Paraffin-NBP2-56042-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIST1H1T antibody NBP2-56042 (A) shows similar protein distribution across tissues to independent antibody NBP2-57668 (B).](http://images.novusbio.com/fullsize2/Histone-H1T-Antibody-Immunohistochemistry-Paraffin-NBP2-56042-img0002.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/fullsize2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize2/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/fullsize2/Histone H2a Antibody-Western Blot-NB100-56346-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: H2A Antibody (4C10) [H00008337-M01] - Analysis of monoclonal antibody to HIST2H2AA3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/H2A Antibody (4C10)-Immunocytochemistry-Immunofluorescence-H00008337-M01-img0003.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on human brain lysate.](http://images.novusbio.com/fullsize2/HOMER1-Antibody-Western-Blot-NBP1-44999-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HOMER1 Antibody [NBP1-44999] - Detection of HOMER1 (Green) in Hela cells using NBP1-44999. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize2/HOMER1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44999-img0007.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on mouse brain lysate.](http://images.novusbio.com/fullsize2/HOMER1-Antibody-Western-Blot-NBP1-44999-img0008.jpg)
![Immunohistochemistry-Paraffin: Acrosin Antibody [NBP2-14260] - Staining in human testis and prostate tissues using anti-ACR antibody. Corresponding ACR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Acrosin-Antibody-Immunohistochemistry-Paraffin-NBP2-14260-img0005.jpg)
![Immunohistochemistry-Paraffin: Acrosin Antibody [NBP2-14260] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize2/Acrosin-Antibody-Immunohistochemistry-Paraffin-NBP2-14260-img0003.jpg)
![Immunohistochemistry-Paraffin: Acrosin Antibody [NBP2-14260] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/Acrosin-Antibody-Immunohistochemistry-Paraffin-NBP2-14260-img0004.jpg)
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/fullsize/HIST1H4F Overexpression Lysate (Adult Normal)-Western Blot-NBP2-07996-img0001.jpg)
![Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.](http://images.novusbio.com/fullsize2/H4-n-Antibody-(3E7H2)-Western-Blot-NBP2-52486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize2/H4-n-Antibody-(3E7H2)-Immunohistochemistry-NBP2-52486-img0003.jpg)
![Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/H4-n-Antibody-(3E7H2)-Immunohistochemistry-Paraffin-NBP2-52486-img0005.jpg)
![Western Blot: H1F0 Antibody [NBP2-16772] - HepG2 whole cell extracts and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) at a dilution of 1:10000 and developed with Trident Sharp-ECL.](http://images.novusbio.com/fullsize2/H1F0-Antibody-Western-Blot-NBP2-16772-img0015.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50](http://images.novusbio.com/fullsize2/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0011.jpg)
![Western Blot: H1F0 Antibody [NBP2-16772] - Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize2/H1F0-Antibody-Western-Blot-NBP2-16772-img0012.jpg)
![Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - Anti-HRH4 / Histamine H4 Receptor antibody IHC of human Ovary, Carcinoma and Inflammatory Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize2/Histamine-H4-R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0002.jpg)
![Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - IHC analysis of Lymph node using this antibody at 12 ug/ ml.](http://images.novusbio.com/fullsize2/Histamine-H4-R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0001.jpg)
![Western Blot: CCDC6 Antibody [NBP1-85351] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/CCDC6-Antibody-Western-Blot-NBP1-85351-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC6 Antibody [NBP1-85351] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CCDC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85351-img0013.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0020.jpg)
![Western Blot: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis using SMCP mAb against HEK293 (1) and SMCP (AA: FULL(1-116))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/SMCP-Antibody-(5C10D8)-Western-Blot-NBP2-37253-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis of Hela cells using SMCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize2/SMCP-Antibody-(5C10D8)-Immunocytochemistry-Immunofluorescence-NBP2-37253-img0004.jpg)
![Western Blot: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis using SMCP mAb against human SMCP (AA: FULL(1-116)) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize2/SMCP-Antibody-(5C10D8)-Western-Blot-NBP2-37253-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-4 Antibody [NBP1-90093] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Septin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90093-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize2/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0012.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SEPT4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0009.jpg)
