![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunocytochemistry-Immunofluorescence-NBP2-45184-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P |
| Clone | HH1/957 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Recombinant full-length human H1F0 Histone H1.0 protein |
| Localization | Nuclear |
| Marker | Pan Nuclear Marker |
| Specificity | Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H1-1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C. |
| Buffer | 1mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: 293 Lane 3: MCF-7](http://images.novusbio.com/images2/Histone-H1.2-Antibody-(JU43-48)-Western-Blot-NBP2-75932-img0001.jpg)
![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.](http://images.novusbio.com/images2/Histone-H1.2-Antibody-(JU43-48)-Western-Blot-NBP2-75932-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-H1.2-Antibody-(JU43-48)-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H1.5 Antibody [NBP3-17162] - Staining of human tonsil shows strong nuclear positivity in germinal- and non germinal center cells.](http://images.novusbio.com/images2/Histone-H1.5-Antibody-Immunohistochemistry-Paraffin-NBP3-17162-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-H1.4-[p-Thr17]-Antibody-(SR38-03)-Immunocytochemistry-Immunofluorescence-NBP2-67313-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Histone-H1.4-[p-Thr17]-Antibody-(SR38-03)-Immunohistochemistry-Paraffin-NBP2-67313-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H1.4 [p Thr17] Antibody (SR38-03) [NBP2-67313] - Analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Histone-H1.4-[p-Thr17]-Antibody-(SR38-03)-Immunohistochemistry-Paraffin-NBP2-67313-img0003.jpg)
![Western Blot: Histone H1.3 Overexpression Lysate (Adult Normal) [NBL1-11554] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H1.3.](http://images.novusbio.com/images/Histone-H1.3-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-11554-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human testis shows nuclear positivity in ductus seminiferous.](http://images.novusbio.com/images2/Histone-H1T-Antibody-Immunohistochemistry-Paraffin-NBP2-56042-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIST1H1T antibody NBP2-56042 (A) shows similar protein distribution across tissues to independent antibody NBP2-57668 (B).](http://images.novusbio.com/images2/Histone-H1T-Antibody-Immunohistochemistry-Paraffin-NBP2-56042-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human kidney.](http://images.novusbio.com/images2/Histone-H1T-Antibody-Immunohistochemistry-Paraffin-NBP2-56042-img0003.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0002.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: H2A Antibody (4C10) [H00008337-M01] - Analysis of monoclonal antibody to HIST2H2AA3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/H2A-Antibody-(4C10)-Immunocytochemistry-Immunofluorescence-H00008337-M01-img0003.jpg)
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/images/HIST1H4F-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-07996-img0001.jpg)
![ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-ELISA-NBP2-52486-img0008.jpg)
![Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Western-Blot-NBP2-52486-img0009.jpg)
![Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Flow-Cytometry-NBP2-52486-img0001.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50](http://images.novusbio.com/images2/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0010.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50](http://images.novusbio.com/images2/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0011.jpg)
![Western Blot: H1F0 Antibody [NBP2-16772] - Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000.](http://images.novusbio.com/images2/H1F0-Antibody-Western-Blot-NBP2-16772-img0012.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0009.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0010.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0012.jpg)
![ELISA: SMCP Antibody (5C10D8) [NBP2-37253] - Black line: Control Antigen (100 ng); Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/images2/SMCP-Antibody-(5C10D8)-ELISA-NBP2-37253-img0001.jpg)
![Western Blot: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis using SMCP mAb against human SMCP (AA: FULL(1-116)) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/images2/SMCP-Antibody-(5C10D8)-Western-Blot-NBP2-37253-img0002.jpg)
![Western Blot: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis using SMCP mAb against HEK293 (1) and SMCP (AA: FULL(1-116))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/SMCP-Antibody-(5C10D8)-Western-Blot-NBP2-37253-img0003.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Chiara Chiereghin |
Immunofluorescence | Human | 03/23/2018 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Diseases for Histone H1 Antibody (NBP2-45184)Discover more about diseases related to Histone H1 Antibody (NBP2-45184).
| Pathways for Histone H1 Antibody (NBP2-45184)View related products by pathway.
|
Research Areas for Histone H1 Antibody (NBP2-45184)Find related products by research area.
|
|
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post. |
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| Chiara Chiereghin 03/23/2018 |
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| Application: | Immunofluorescence | |
| Species: | Human |
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0008.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).")
![Flow Cytometry: Histone H1 Antibody (HH1/957) [NBP2-45184] - Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Monoclonal Antibody (HH1/957) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Flow-Cytometry-NBP2-45184-img0009.jpg "Flow Cytometry: Histone H1 Antibody (HH1/957) [NBP2-45184] - Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Monoclonal Antibody (HH1/957) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunocytochemistry-Immunofluorescence-NBP2-45184-img0005.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0001.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0002.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0004.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0003.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0007.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.](http://images.novusbio.com/images2/Histone-H1-Antibody-(HH1-957)-Immunocytochemistry-Immunofluorescence-NBP2-45184-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/images2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/images2/Histone-H1-Antibody-(HH1-957)-Immunohistochemistry-Paraffin-NBP2-45184-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HOMER1 Antibody [NBP1-44999] - Detection of HOMER1 (Green) in Hela cells using NBP1-44999. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/images2/HOMER1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44999-img0007.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on mouse brain lysate.](http://images.novusbio.com/images2/HOMER1-Antibody-Western-Blot-NBP1-44999-img0008.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on human brain lysate.](http://images.novusbio.com/images2/HOMER1-Antibody-Western-Blot-NBP1-44999-img0009.jpg)
![Immunohistochemistry-Paraffin: Acrosin Antibody [NBP2-14260] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/images2/Acrosin-Antibody-Immunohistochemistry-Paraffin-NBP2-14260-img0003.jpg)
![Immunohistochemistry-Paraffin: Acrosin Antibody [NBP2-14260] - Staining of human testis shows high expression.](http://images.novusbio.com/images2/Acrosin-Antibody-Immunohistochemistry-Paraffin-NBP2-14260-img0004.jpg)
![Immunohistochemistry-Paraffin: Acrosin Antibody [NBP2-14260] - Staining in human testis and prostate tissues using anti-ACR antibody. Corresponding ACR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Acrosin-Antibody-Immunohistochemistry-Paraffin-NBP2-14260-img0005.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0003.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0004.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0005.jpg)
