Reactivity | Hu, Mu, RtSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC, IHC-P |
Clone | HH1/957 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Immunogen | Recombinant full-length human H1F0 Histone H1.0 protein |
Localization | Nuclear |
Marker | Pan Nuclear Marker |
Specificity | Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
Isotype | IgG2a Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | H1-1 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Reviewed Applications |
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Storage | Store at 4C. |
Buffer | 1mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Chiara Chiereghin |
Immunofluorescence | Human | 03/23/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
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Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post. |
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Chiara Chiereghin 03/23/2018 |
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Application: | Immunofluorescence | |
Species: | Human |