Histone H1 Antibody (HH1/957)

Western Blot: Histone H1 Antibody (HH1/957) [NBP2-45184] - Western Blot Analysis of human heart tissue lysate using Histone H1 Antibody (HH1/957).

Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Antibody (HH1/957).

Flow Cytometry: Histone H1 Antibody (HH1/957) [NBP2-45184] - Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Monoclonal Antibody (HH1/957) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: HH1/957
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Histone H1 Antibody (HH1/957) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47754)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Immunogen: Recombinant full-length human H1F0 Histone H1.0 protein
• Localization: Nuclear
• Marker: Pan Nuclear Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H1-1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 1mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Histone H1 Antibody (HH1/957)

• H1 histone family, member 1
• H1.1
• H1A
• H1F1HIST1
• histone 1, H1a
• histone cluster 1, H1a
• histone H1.1
• MGC126642
• MGC138345

Background

Histone H1 is the linker protein that is 194 amino acids long, weights approximately 21 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1 has a shorter isoform measuring 177 amino acids long and weighing approximately 19 kDa as well. Current studies are being done on several diseases and disorders including paratyphoid fever, hemochromatosis, breast cancer, embryonal carcinoma, prostatitis, carcinoma, and neuronitis. This protein has also been shown to have interactions with ADPRH, CDK2, CDK7, CTCFL, and DOT1L in pathways such as the histone modification, DNA replication, chromosome condensation, PKA signaling, granzyme-A, and apoptosis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Histone H1 Antibody (NBP2-45184) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Chiara Chiereghin IF Human 03/23/2018

Summary

• Application: Immunofluorescence
• Sample Tested: Cell culture
• Species: Human
• Lot: 3005-3P170404

Bioinformatics

• Gene Symbol: H1-1
• Uniprot:
  • P07305(Human)