Reactivity | Hu, Mu, RtSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC |
Clone | HH1/957 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47754) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
Immunogen | Recombinant full-length human H1F0 Histone H1.0 protein |
Localization | Nuclear |
Marker | Pan Nuclear Marker |
Isotype | IgG2a Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | H1-1 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Reviewed Applications |
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Storage | Store at 4C. |
Buffer | 1mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Chiara Chiereghin |
IF | Human | 03/23/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
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