Cyclin A2 Antibody

Images

 
Biological Strategies: Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 ...read more
Immunocytochemistry/ Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by Cyclin A2 antibody diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:500 dilution.
Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Cyclin A2 Antibody [NBP1-31330] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug HeLa whole cell lysate/extract, C. 30 ug A37C whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.
Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

Cyclin A2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCNA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP1-31330 in the following applications:

Publications
Read Publications using
NBP1-31330 in the following applications:

  • WB
    2 publications

Reactivity Notes

Pig (89%), Rabbit (83%), Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Cyclin A2 Antibody

  • CCN1CCNAcyclin-A
  • CCNA
  • CCNA2
  • Cyclin A2
  • Cyclin-A
  • cyclin-A2

Background

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cyclin A2 Antibody (NBP1-31330)(4)

Reviews for Cyclin A2 Antibody (NBP1-31330) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP1-31330:
Filter by Applications
WB
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol
Immunohistochemistry-Paraffin Cyclin A2 NBP1-31330
Enlarge
reviewed by:
Anonymous
IHC-P Human 10/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested293T cell line
SpeciesHuman
Western Blot Cyclin A2 NBP1-31330
Enlarge
reviewed by:
Anonymous
WB Human 09/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio700 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody (Cell Signaling)
Secondary Manufacturer Cat#7074
Secondary Concentration20,000

Details

Detection NotesCaptured using X-rays (10 sec exposure) with Phototope-HRP Western Blot Detection System

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyclin A2 Antibody (NBP1-31330) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cyclin A2 Antibody (NBP1-31330)

Discover related pathways, diseases and genes to Cyclin A2 Antibody (NBP1-31330). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Cyclin A2 Antibody (NBP1-31330)

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PTMs for Cyclin A2 Antibody (NBP1-31330)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
10/04/2015
Application: WB
Species: Human

Anonymous
10/02/2015
Application: IHC-P
Species: Human

Anonymous
09/19/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CCNA2