CXCR4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-716] - Anti-CXCR4 Inhibition of SDF-Induced Activation DU145
Immunohistochemistry: CXCR4 Antibody [NB100-716] - Immunohistochemistry on paraffin sections of human spleen

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CXCR4 Antibody Summary

Immunogen
Synthetic peptide (TEEMGSGDYDSMKEPCFREENANFNKC) corresponding to N-terminal extracellular domain of human CXCR4 receptor.
Localization
Cell Membrane
Specificity
Peptide sequence is < 50% identical to other human chemokine receptors in this region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250
  • ELISA 1:25000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Positive Control
Blood Leukocyte Lysate (NB820-59174)
Spleen Lysate (NB820-59259)
Publications
Read Publications using
NB100-716 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM KHPO4, 0.14M NaCl and 1.0 mg/ml BSA
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CXCR4 Antibody

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LCR1
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3RWHIM
  • NPYR
  • NPYY3R
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor

Background

CXCR4 is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The a chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. The amino terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR4 Antibody (NB100-716)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CXCR4 Antibody (NB100-716). (Showing 1 - 1 of 1 FAQs).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CXCR4 Products

Bioinformatics Tool for CXCR4 Antibody (NB100-716)

Discover related pathways, diseases and genes to CXCR4 Antibody (NB100-716). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for CXCR4 Antibody (NB100-716)

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Blogs on CXCR4.

LAMP2: Protector of the lysosome
LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.

CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.

Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.

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Bioinformatics

Gene Symbol CXCR4
Entrez
Uniprot