CXCR3 Antibody (5C10E6)

Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mAb against HEK293 (1) and CD183 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of paraffin-embedded esophageal cancer tissues using CD183 mouse mAb with DAB staining.

Flow Cytometry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of Ramos cells using CD183 mouse mAb (green) and negative control (red).

Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mAb against human CD183 (AA: extra mix) recombinant protein. (Expected MW is 38.3 kDa)

Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mouse mAb against Hela (1) and L-02 (2) cell lysate.

Immunohistochemistry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of paraffin-embedded cervical cancer tissues using CD183 mouse mAb with DAB staining.

Flow Cytometry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of Jurkat cells using CD183 mouse mAb (green) and negative control (red).

ELISA: CXCR3 Antibody (5C10E6) [NBP2-61681] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-P
• Clone: 5C10E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR3 Antibody (5C10E6) Summary

• Immunogen: Purified recombinant fragment of human CD183 (AA: extra mix) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
• Theoretical MW: 40.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for CXCR3 Antibody (5C10E6)

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCR3