CXCR3 Antibody (220803) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CXCR3 Monoclonal Antibody (Catalog # MAB1685) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 220803
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR3 Antibody (220803) [Unconjugated] Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with mouse CXCR3
  Met1-Leu367
  Accession # AAC40163
• Specificity: Detects mouse CXCR3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR3

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR3 Antibody (220803) [Unconjugated]

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

CXCR3 is a G protein coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10) and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR3 Antibody (MAB1685)(11)

Publications using MAB1685

• CM Harpur, Y Kato, ST Dewi, S Stankovic, DN Johnson, S Bedoui, PG Whitney, MH Lahoud, I Caminschi, WR Heath, AG Brooks, T Gebhardt Classical Type 1 Dendritic Cells Dominate Priming of Th1 Responses to Herpes Simplex Virus Type 1 Skin Infection J. Immunol., 2018;0(0):. 2018 [PMID: 30598513] (Flow Cytometry, Mouse)
• A DiPiazza, N Laniewski, A Rattan, DJ Topham, J Miller, AJ Sant CD4 T cell epitope specificity and cytokine potential are preserved as cells transition from the lung vasculature to lung tissue following influenza infection J. Virol., 2018;0(0):. 2018 [PMID: 29669836] (Flow Cytometry, Mouse)
• The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells Nat. Immunol, 2016;0(0):. 2016 [PMID: 27992400] (Flow Cytometry, Mouse)
• SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin J. Immunol, 2016;196(6):2514-25. 2016 [PMID: 26851219] (Flow Cytometry, Mouse)
• Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery. J Leukoc Biol, 2015;97(3):583-98. 2015 [PMID: 25583577] (Flow Cytometry, Mouse)
• Yoo J, Braciale T IL-21 promotes late activator APC-mediated T follicular helper cell differentiation in experimental pulmonary virus infection. PLoS ONE, 2014;9(9):e105872. 2014 [PMID: 25251568] (Flow Cytometry, Mouse)
• Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice. PLoS ONE, 2013;8(3):e58140. 2013 [PMID: 23520491]
• Guarda G, Dostert C, Staehli F, Cabalzar K, Castillo R, Tardivel A, Schneider P, Tschopp J T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes. Nature, 2009;460(7252):269-73. 2009 [PMID: 19494813] (Flow Cytometry, Mouse)
• Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells. Nat. Immunol., 2007;8(7):743-52. 2007 [PMID: 17529983] (Flow Cytometry, Mouse)
• Matsuda JL, George TC, Hagman J, Gapin L Temporal dissection of T-bet functions. J. Immunol., 2007;178(6):3457-65. 2007 [PMID: 17339440] (Flow Cytometry, Mouse)
• Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection. J. Virol., 2007;81(3):1241-50. 2007 [PMID: 17108043] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCR3
• Uniprot:
  • AAC40163()