CXCL13/BLC/BCA-1 Antibody (PSH04-34)

Western Blot: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Western blot analysis of CXCL13/BLC/BCA-1 on different lysates with Rabbit anti-CXCL13/BLC/BCA-1 antibody (NBP3-32234) at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-CXCL13 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 13 kDa Observed band size: 12/13 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32234) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CXCL13/BLC/BCA-1 antibody (NBP3-32234) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32234) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Immunocytochemistry analysis of HeLa transfacted with or without CXCL13/BLC/BCA-1 cells labeling CXCL13/BLC/BCA-1 with Rabbit anti-CXCL13/BLC/BCA-1/BLC/BCA-1 antibody (NBP3-32234) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL13 antibody (NBP3-32234) at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: PSH04-34
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CXCL13/BLC/BCA-1 Antibody (PSH04-34) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human CXCL13/BLC/BCA-1 aa 1-109 / 109. (Uniprot: O43927)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CXCL13
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:1000
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CXCL13/BLC/BCA-1 Antibody (PSH04-34)

• ANGIE
• ANGIE2
• B cell-attracting chemokine 1
• B lymphocyte chemoattractant
• BCA1
• BCA-1
• BCA1B-cell chemoattractant
• BCA-1CXC chemokine BLC
• B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
• BLC
• BLCSmall-inducible cytokine B13
• BLR1L
• B-lymphocyte chemoattractant
• chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
• chemokine (C-X-C motif) ligand 13
• C-X-C motif chemokine 13
• CXCL13
• SCYB13
• SCYB13B-cell-attracting chemokine 1
• small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

Plasmid: pCMV-CXCL13 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCL13