CXCL13/BLC/BCA-1 Products

470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/ BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND₅₀ is typically 20-50 µg/mL." class="big_lightbox" target="_blank">

NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

470-BC) was serially diluted and captured by Rat Anti-Mouse CXCL13/BLC/BCA‑1 Monoclonal Antibody (Catalog # MAB470) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox" target="_blank">

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

AF801) at 5ug/mL with 1-hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. " class="big_lightbox" target="_blank">

801-CX) was serially diluted and captured by Mouse Anti-Human CXCL13/BLC/BCA‑1 Monoclonal Antibody (Catalog # MAB801) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">