CXCL12/SDF-1 beta Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL12/SDF-1 beta Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CXCL12/SDF‑1 beta
  Lys22-Met93
  Accession # P48061
• Specificity: Detects human CXCL12/SDF-1 beta in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant (rh) SDF‑1 alpha and recombinant mouse SDF‑1 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human/Mouse CXCL12/SDF-1 Antibody (Catalog # MAB350)
  ELISA Detection: Human CXCL12/SDF-1 beta Biotinylated Antibody (Catalog # BAF351)
  Standard:Recombinant Human/Feline CXCL12/SDF-1 beta aa 22-93 (Catalog # 351-FS)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL12/SDF-1 beta Antibody [Biotin]

• CXCL12/SDF-1 beta
• hSDF-1beta
• SDF 1beta
• SDF1 beta
• SDF1b

Background

CXCL12, also known as SCYB12, PBSF and SDF-1 beta , is an 8.3 kDa, heparin-binding member of the CXC (or alpha-) family of chemokines (1, 2). Feline CXCL12( beta ) is synthesized as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region (3). The mature molecule exhibits a typical three antiparallel beta -strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa’s 1 - 8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation (4). The C-terminus is likely associated with heparin binding (5). SDF-1 beta circulates and undergoes proteolytic processing. CD26 will remove the first two N-terminal amino acids, possibly creating a reduced-activity chemokine (5, 6). In addition to the beta -isoform, alternate splicing of the feline SDF-1 gene generates an alpha -isoform. The alpha isoform is identical to SDF-1 beta , but shorter by four aa’s at the C-terminus (3). Although alpha - and beta -isoforms show similar activity, SDF-1 alpha is differentially processed, and different cells secrete the two isoforms (5, 7). Mature feline SDF-1 beta is 96%, 97% and 100% aa identical to rat, mouse and human SDF-1 beta , respectively. Human (and by inference, feline) SDF-1 is active on mouse cells. SDF-1 alpha and beta are reported to be monomers at neutral pH and physiologic ionic strength (4). SDF-1 alpha is also reported to form dimers in the presence of heparansulfate (8). On the cell surface, this may well facilitate SDF-1 interaction with its two receptors, CXCR4 and syndecan-4 (9). Heparin sulfate is known to protect SDF-1 from proteolysis, and CXCR4 exists constitutively as a dimer (9 - 11). Among its many functions, CXCL12 is known to influence lymphopoiesis, regulate patterning and cell number of neural progenitors, and promote angiogenesis (12, 13). It also enhances the survival of myeloid progenitor cells.

1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
2. Rollins, B.J. (1997) Blood 90:909.
3. Nishimura, Y. et al. (1998) Eur. J. Immunogenet. 25:303.
4. Crump, M.P. et al. (1997) EMBO J. 16:6996.
5. Sierra, M.D. L. et al. (2004) Blood 103:2452.
6. Davis, D.A. et al. (2005) Blood 105:4561.
7. Stumm, R.K. et al. (2002) J. Neurosci. 22:5865.
8. Veldkamp, C.T. et al. (2005) Protein Sci. 14:1071.
9. Charnaux, N. et al. (2005) FEBS J. 272:1937.
10. Percherancier, Y. et al. (2005) J. Biol. Chem. 280:9895.
11. Babcock, G.J. et al. (2003) J. Biol. Chem. 278:3378.
12. Klein, R.S. et al. (2004) Trends Immunol. 25:306.
13. Salcedo, R. and J.J. Oppenheim (2003) Microcirculation 10:359.
14. Broxmeyer, H.E. et al. (2003) J. Leukoc. Biol. 73:630.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL12/SDF-1 beta Antibody (BAF351)(5)

Publications using BAF351

• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)
• Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta. Glia, 2006-11-01;54(6):619-29. 2006-11-01 [PMID: 16944452] (ELISA Development, Human)
• Infantino S, Moepps B, Thelen M Expression and regulation of the orphan receptor RDC1 and its putative ligand in human dendritic and B cells. J. Immunol., 2006-02-15;176(4):2197-207. 2006-02-15 [PMID: 16455976] (ELISA Development, Human)
• Levesque JP, Liu F, Simmons PJ, Betsuyaku T, Senior RM, Pham C, Link DC Characterization of hematopoietic progenitor mobilization in protease-deficient mice. Blood, 2004-03-09;104(1):65-72. 2004-03-09 [PMID: 15010367] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CXCL12
• Uniprot:
  • P48061()